复制蛋白A与含有氧化嘧啶碱基的单链DNA的结合活性。

Binding activity of replication protein A to single-stranded DNA containing oxidized pyrimidine base.

作者信息

Kasama T, Ono A, Izuta S

机构信息

Faculty of Science, Kumamoto University, Kumamoto 860-8555, Japan.

出版信息

Nucleic Acids Res Suppl. 2001(1):167-8. doi: 10.1093/nass/1.1.167.

DOI:10.1093/nass/1.1.167

PMID:12836317

Abstract

To obtain the information for the role of replication protein A (RPA) on the detection of oxidized lesion in the single-stranded DNA, the binding preference of RPA purified from Xenopus egg lysate against the oligonucleotide containing one of three kinds of oxidized thymine residues, 5-formyluracil, 5-hydroxymethyluracil and 5-(1,2-dihydroxyethyl)uracil, was studied by the gel shift assay. Results of competition assay indicate that RPA preferentially binds to the oligonucleotide containing these oxidized thymine residues than the undamaged DNA.

摘要

为了获取复制蛋白A（RPA）在单链DNA中氧化损伤检测方面的作用信息，通过凝胶迁移试验研究了从非洲爪蟾卵裂解物中纯化的RPA对含有三种氧化胸腺嘧啶残基之一（5-甲酰基尿嘧啶、5-羟甲基尿嘧啶和5-(1,2-二羟乙基)尿嘧啶）的寡核苷酸的结合偏好。竞争试验结果表明，与未损伤的DNA相比，RPA优先结合含有这些氧化胸腺嘧啶残基的寡核苷酸。