在福尔马林固定、石蜡包埋组织中用单克隆抗体鉴定猪巨噬细胞。
Identification of porcine macrophages with monoclonal antibodies in formalin-fixed, paraffin-embedded tissues.
作者信息
Domenech Nieves, Rodríguez-Carreño M Pilar, Filgueira Purificación, Alvarez Belén, Chamorro Sonia, Domínguez Javier
机构信息
Unidad de Investigación, Complejo Hospitalario "Juan Canalejo", 15006 A Coruña, Spain.
出版信息
Vet Immunol Immunopathol. 2003 Jul 15;94(1-2):77-81. doi: 10.1016/s0165-2427(03)00084-9.
Here we report the successful use of monoclonal antibodies (mAbs) BL1H7, BA1C11 (anti-SWC3) and 4E9 for immunohistochemical identification of macrophages in formalin-fixed, paraffin-embedded porcine tissues. Retrieval of antigen reactivity was achieved by heating the slides in a domestic pressure cooker, which makes the technique suitable for the routine laboratory. This method allows to perform retrospective studies in routinely processed tissues and may be useful to investigate the role of macrophages in different pathological processes.
在此，我们报告了单克隆抗体（mAb）BL1H7、BA1C11（抗SWC3）和4E9在福尔马林固定、石蜡包埋的猪组织中用于巨噬细胞免疫组织化学鉴定的成功应用。通过在家庭压力锅中加热载玻片实现抗原反应性的恢复，这使得该技术适用于常规实验室。该方法允许对常规处理的组织进行回顾性研究，并且可能有助于研究巨噬细胞在不同病理过程中的作用。
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