对血红蛋白E/E和β地中海贫血/血红蛋白/E患者血液中血红蛋白F进行自动测量。

Automatic measurement of hemoglobin F in blood obtained from patients with hemoglobin E/E and beta-thalassemia/hemoglobin/E.

作者信息

Tatsumi N, Tsuda I, Funahara Y, Bunyaratvej A, Fucharoen S

机构信息

Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.

出版信息

Southeast Asian J Trop Med Public Health. 1992;23 Suppl 2:91-4.

PMID:1284356

Abstract

This paper presents an automated determination of hemoglobin (Hb) F in Hb E/E disease using Hi-Auto A1c. Blood specimens collected in Bangkok were frozen, and sent to Japan by air mail for the determination. The automatically determined values showed a high correlation with the values obtained by the classical alkali denaturation method. Hb E/E cases showed 4.24 +/- 1.75% of Hb F. On the other hand, Hb, Hct, MCV and MCH in the disease samples were lower than in the controls, but higher than those of beta-thalassemia/HbE disease. From the results it was concluded that Hb E/E could be differentiated from beta-thalassemia/HbE by combination of Hb F value and MCH or Hb in CBC.

摘要

本文介绍了使用Hi-Auto A1c自动测定Hb E/E疾病中血红蛋白（Hb）F的方法。在曼谷采集的血液标本被冷冻，然后通过航空邮件送往日本进行测定。自动测定值与经典碱变性法获得的值显示出高度相关性。Hb E/E病例的Hb F为4.24 +/- 1.75%。另一方面，疾病样本中的Hb、Hct、MCV和MCH低于对照组，但高于β地中海贫血/HbE疾病患者。从结果得出结论，通过结合Hb F值和血常规中的MCH或Hb，可以将Hb E/E与β地中海贫血/HbE区分开来。