一种来自菜豆根的氯离子通透通道被整合到平面脂质双分子层中。
A chloride-permeable channel from Phaseolus vulgaris roots incorporated into planar lipid bilayers.
作者信息
Balleza Daniel, Quinto Carmen, Sánchez Federico, Gómez-Lagunas Froylan
机构信息
Departamento de Biologi;a Molecular de Plantas, Instituto de Biotecnologi;a, UNAM, Cuernavaca Morelos 62210, Mexico.
出版信息
Biochem Biophys Res Commun. 2003 Jul 18;307(1):114-8. doi: 10.1016/s0006-291x(03)01128-8.
Ion channels are key participants in physiological processes of plant cells. Here, we report the first characterization of a high conductance, Cl(-)-permeable channel, present in enriched fractions of plasma membranes of bean root cells. The Cl(-) channel was incorporated into planar lipid bilayers and its activity was recorded under voltage clamp conditions. The channel is voltage-dependent, excludes the passage of cations (K(+), Na(+), and Ca(2+)), and is inhibited by micromolar concentrations of Zn(2+). The Cl(-) conductance here characterized represents a previously undescribed channel of plant cells.
离子通道是植物细胞生理过程中的关键参与者。在此，我们报道了一种高电导、Cl(-) 通透通道的首次特性描述，该通道存在于菜豆根细胞质膜的富集组分中。Cl(-) 通道被整合到平面脂双层中，并在电压钳制条件下记录其活性。该通道具有电压依赖性，排除阳离子（K(+)、Na(+) 和 Ca(2+)）的通过，并且受到微摩尔浓度的 Zn(2+) 的抑制。这里所描述的 Cl(-) 电导代表了植物细胞中一种先前未被描述的通道。
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