三氧化二砷诱导NB4细胞中的基因表达谱变化。
Gene expression profile changes in NB4 cells induced by arsenic trioxide.
作者信息
Wang Huai-Yu, Liu Shan-Xi, Zhang Mei
机构信息
Department of Hematology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
出版信息
Acta Pharmacol Sin. 2003 Jul;24(7):646-50.
AIM
To investigate the gene expression profiles of acute promyelocytic leukemia (APL) cell line NB4 treated with arsenic trioxide (As2O3) using cDNA microarray.
METHODS
Two cDNA probes were prepared through reverse transcription from mRNA of NB4 cells treated with or without arsenic trioxide. The probes were labeled with Cy3 and Cy5 fluorescence dyes individually, hybridized with cDNA microarray representing 1003 different human genes, and their fluorescent intensities were scanned. The genes were screened through the analysis of the difference in two gene expression profiles.
RESULTS
The analysis of gene expression profiles indicated that after the treatment of arsenic trioxide (0.5 micromol/L) 3 genes were up-regulated, among which, PSMB6 gene was involved in proteasome degradation pathway, and 18 genes related to RNA processing, protein synthesis, and signal transduction were down-regulated.
CONCLUSION
PSMB6 and ITGB1 genes may be related to the differentiation and/or apoptosis of NB4 cells induced by As2O3.
目的
采用cDNA微阵列技术研究三氧化二砷（As2O3）处理的急性早幼粒细胞白血病（APL）细胞系NB4的基因表达谱。
方法
从经或未经三氧化二砷处理的NB4细胞的mRNA通过逆转录制备两种cDNA探针。探针分别用Cy3和Cy5荧光染料标记，与代表1003个人类不同基因的cDNA微阵列杂交，并扫描其荧光强度。通过分析两个基因表达谱的差异来筛选基因。
结果
基因表达谱分析表明，经三氧化二砷（0.5微摩尔/升）处理后，3个基因上调，其中PSMB6基因参与蛋白酶体降解途径，18个与RNA加工、蛋白质合成和信号转导相关的基因下调。
结论
PSMB6和ITGB1基因可能与As2O3诱导的NB4细胞分化和/或凋亡有关。
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