Zhang Lei, Onda Kouki, Imai Ryozo, Fukuda Ryouichi, Horiuchi Hiroyuki, Ohta Akinori
Laboratory of Cellular Genetics, Department of Biotechnology, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Biochem Biophys Res Commun. 2003 Jul 25;307(2):308-14. doi: 10.1016/s0006-291x(03)01168-9.
A rapid downshift in the growth temperature of Saccharomyces cerevisiae from 30 to 10 degrees C resulted in an increase in transcript levels of the antioxidation genes SOD1 [encoding Cu-Zn superoxide dismutase (SOD)], CTT1 (encoding catalase T), and GSH1 (encoding gamma-glutamylcysteine synthetase). The cellular activities of SOD and catalase were also increased, indicating that the temperature downshift caused an antioxidant response. In support of this, a simultaneous increase in the intracellular level of H(2)O(2) was observed. The level of YAP1 mRNA, encoding a transcription factor critical for the oxidative stress response in this yeast, was also increased by the temperature downshift. However, deletion of YAP1 did not reduce the elevated mRNA levels of the antioxidant genes. This suggests that the temperature downshift-induced increase in the mRNA level of anti-oxidant genes is YAP1-independent.
酿酒酵母的生长温度从30℃迅速降至10℃，导致抗氧化基因SOD1（编码铜锌超氧化物歧化酶[SOD]）、CTT1（编码过氧化氢酶T）和GSH1（编码γ-谷氨酰半胱氨酸合成酶）的转录水平升高。超氧化物歧化酶和过氧化氢酶的细胞活性也增加，表明温度下降引发了抗氧化反应。与此相符的是，观察到细胞内H₂O₂水平同时升高。编码对该酵母氧化应激反应至关重要的转录因子的YAP1 mRNA水平，也因温度下降而升高。然而，YAP1基因的缺失并未降低抗氧化基因升高的mRNA水平。这表明温度下降诱导的抗氧化基因mRNA水平升高不依赖于YAP1。
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