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果蝇单个外胚层前体的原代培养揭示了原肠胚早期内在神经发生和表皮发生能力的背腹预模式。

Primary culture of single ectodermal precursors of Drosophila reveals a dorsoventral prepattern of intrinsic neurogenic and epidermogenic capabilities at the early gastrula stage.

作者信息

Lüer K, Technau G M

机构信息

Institut für Genetik, Zellbiologie, Universität Mainz, Germany.

出版信息

Development. 1992 Oct;116(2):377-85. doi: 10.1242/dev.116.2.377.

Abstract

We have analyzed the development in vitro of individual precursor cells from the presumptive truncal segmental ectoderm of the Drosophila embryo to study the intrinsic component in the determination of cell fate. For each cultured cell, the original position within as well as the developmental stage of the donor embryo were known. Cells removed from the ventral neurogenic region develop neural clones. Cells from the dorsal ectoderm and from the dorsalmost part of the ventral neurogenic ectoderm develop epidermal clones. These two classes of clones differ with respect to their division pattern, adhesiveness, cell morphologies and the expression of cell-specific markers. Mixed neural/epidermal clones were obtained from a fraction of precursors at almost all dorsoventral sites. We conclude that, at the onset of gastrulation, precursor cells of the truncal segmental ectoderm already have the capability to develop as either neuroblasts or epidermoblasts in the absence of further cell interactions. At the same time, positional cues distributed along the dorsoventral axis equip precursors with intrinsic preferences towards the neural or epidermal fate, thus defining a prepattern of high neurogenic preferences ventrally, and high epidermogenic preferences dorsally. It is likely that this prepattern is involved in defining the extent of the ventral neurogenic and dorsal epidermogenic regions of the ectoderm. The roles of intrinsic capabilities versus extrinsic influences in the regulation of the characteristic pattern of segregation of the two lineages are discussed.

摘要

我们分析了果蝇胚胎假定躯干节段外胚层中单个前体细胞的体外发育情况,以研究细胞命运决定中的内在因素。对于每个培养的细胞,其在供体胚胎中的原始位置以及发育阶段都是已知的。从腹侧神经源区域移除的细胞会发育成神经克隆。来自背侧外胚层和腹侧神经源外胚层最背侧部分的细胞会发育成表皮克隆。这两类克隆在分裂模式、黏附性、细胞形态以及细胞特异性标记物的表达方面存在差异。几乎在所有背腹位点,从一部分前体细胞中都获得了混合神经/表皮克隆。我们得出结论,在原肠胚形成开始时,躯干节段外胚层的前体细胞在没有进一步细胞相互作用的情况下已经具备发育成神经母细胞或表皮母细胞的能力。同时,沿背腹轴分布的位置线索使前体细胞对神经或表皮命运具有内在偏好,从而确定了腹侧具有高神经源性偏好、背侧具有高表皮源性偏好的预模式。这种预模式可能参与确定外胚层腹侧神经源区域和背侧表皮源区域的范围。本文还讨论了内在能力与外在影响在调控这两个谱系特征性分离模式中的作用。

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