Chandler Wayne L, Ferrell Chris, Lee Joo, Tun Theingi, Kha Hien
Dept of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195, USA.
Am J Clin Pathol. 2003 Jul;120(1):34-9. doi: 10.1309/C8T8-YNB4-G3W4-5PRF.
We compared 1-stage clot-based, chromogenic, and immunoassay methods for measuring factor VIII in plasma with a focus on the measurement of elevated levels of factor VIII. The chromogenic assay showed the best interassay imprecision for factor VIII levels near 150 IU/dL. The best correlation was between the 1-stage clot-based and chromogenic factor VIII assays (r2 = 0.934), and the lowest correlation was between the 1-stage clot-based and antigenic factor VIII assays (r2 = 0.821). The presence of heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors in the sample resulted in major interference in the 1-stage clot-based assay but not the chromogenic or antigenic factor VIII assays. Overall, the chromogenic factor VIII activity assay was the optimal method, showing good precision, the best overall correlation with other assays, and no interference from heparin, low-molecular-weight heparin, lepirudin, or lupus inhibitors.
我们比较了用于测量血浆中凝血因子VIII的一步法凝血检测、发色底物法和免疫测定法，重点是对升高的凝血因子VIII水平的测量。发色底物法在凝血因子VIII水平接近150 IU/dL时显示出最佳的批间精密度。一步法凝血检测与发色底物法检测凝血因子VIII之间的相关性最佳（r2 = 0.934），而一步法凝血检测与抗原性凝血因子VIII检测之间的相关性最低（r2 = 0.821）。样本中存在肝素、低分子量肝素、水蛭素或狼疮抑制剂会对一步法凝血检测产生重大干扰，但不会干扰发色底物法或抗原性凝血因子VIII检测。总体而言，发色底物法凝血因子VIII活性检测是最佳方法，显示出良好的精密度，与其他检测方法的总体相关性最佳，并且不受肝素、低分子量肝素、水蛭素或狼疮抑制剂的干扰。
