1,2 -丙二醇与乙二醇用于慢速冷冻小鼠受精卵及其后续发育的比较
Comparison of 1,2-propanediol and ethylene glycol for cryopreservation of slow-cooled mouse zygotes and their subsequent development.
作者信息
Bafrani Hassan Hassani, Salsabil Naser, Pasbakhsh Parichehr, Hassani Hossein, Movahedin Mansoureh, Al-tarihi Taghi, Akbari Firoozeh, Keshavarz Mansour
机构信息
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
J Assist Reprod Genet. 2003 Jun;20(6):234-40. doi: 10.1023/a:1024159512353.
PURPOSE
This study was conducted on cryoprotective activity of ethylene glycol (ETG) and propanediol (PROH) on cleavage rate of mouse zygotes.
METHODS
Mouse oocytes were excised from fallopian tube of gonadotropin-treated mice, then inseminated with spermatozoa. After 16.5-17.5 h, zygotes were randomly allocated into three groups; control, toxicity, and frozen. In the latter, zygotes were slowly cooled with ETG and PROH similar to those used for human embryo cryopreservation. The survived zygotes cultured for 120 h and their later stages of development were compared with nonfrozen embryos.
RESULTS
The toxicity test showed that no differences were observed in cleavage rate between exposed and nonexposed embryos. The survival and expanded hatching blastocyst rate of embryos frozen with PROH was significantly better than with ETG (92.8 vs. 58.2% and 68.2 vs. 39.1%, respectively).
CONCLUSIONS
ETG does not appear to be a good alternative to the classical PROH for freezing of mouse zygotes.
目的
本研究旨在探讨乙二醇（ETG）和丙二醇（PROH）对小鼠受精卵卵裂率的冷冻保护活性。
方法
从经促性腺激素处理的小鼠输卵管中取出卵母细胞，然后与精子受精。16.5 - 17.5小时后，将受精卵随机分为三组：对照组、毒性组和冷冻组。在冷冻组中，受精卵用与人类胚胎冷冻保存相同的ETG和PROH缓慢冷却。将存活的受精卵培养120小时，并将其后期发育阶段与未冷冻的胚胎进行比较。
结果
毒性试验表明，暴露组和未暴露组胚胎的卵裂率没有差异。用PROH冷冻的胚胎的存活率和扩张孵化囊胚率明显优于用ETG冷冻的胚胎（分别为92.8%对58.2%和68.2%对39.1%）。
结论
对于小鼠受精卵的冷冻，ETG似乎不是经典的PROH的良好替代品。
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