Rafati Sima, Nakhaee Alireza, Taheri Tahere, Ghashghaii Andishe, Salmanian Ali Hatef, Jimenez Maribel, Mohebali Mehdi, Masina Slavica, Fasel Nicolas
Department of Immunology, Pasteur Institute of Iran, Tehran, Iran.
Exp Parasitol. 2003 Mar-Apr;103(3-4):143-51. doi: 10.1016/s0014-4894(03)00097-3.
In this study, the mature domains of type I (CPB) and type II (CPA) cysteine proteinases (CPs) of Leishmania infantum were expressed and their immunogenic properties defined using sera from active and recovered cases of human visceral leishmaniasis and sera from infected dogs. Immunoblotting and ELISA analysis indicated that a freeze/thaw extract of parasite antigens showed similar and intensive recognition in both active cases of human and dog sera but lower recognition in recovered human individuals. The total IgG of actively infected human sera was higher than in recovered cases when rCPs were used as antigen. In contrast to dog sera, both active and recovered human cases have higher recognition toward rCPB than rCPA. Furthermore, the asymptomatic dogs in contrast to the symptomatic cases exhibited specific lymphocyte proliferation to both crude antigens and rCPs.
在本研究中，表达了婴儿利什曼原虫I型（CPB）和II型（CPA）半胱氨酸蛋白酶（CPs）的成熟结构域，并使用来自人类内脏利什曼病活动期和康复期病例的血清以及感染犬的血清来确定它们的免疫原性。免疫印迹和ELISA分析表明，寄生虫抗原的冻融提取物在人类和犬血清的活动期病例中显示出相似且强烈的识别，但在康复的人类个体中识别较低。当使用重组CPs作为抗原时，活动性感染人类血清的总IgG高于康复病例。与犬血清不同，活动期和康复期的人类病例对重组CPB的识别高于重组CPA。此外，与有症状病例相比，无症状犬对粗抗原和重组CPs均表现出特异性淋巴细胞增殖。
