在天蓝色链霉菌和毕赤酵母中表达的细胞外大肠杆菌AppA植酸酶的比较。
Comparison of extracellular Escherichia coli AppA phytases expressed in Streptomyces lividans and Pichia pastoris.
作者信息
Stahl Chad H, Wilson David B, Lei Xin Gen
机构信息
Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
出版信息
Biotechnol Lett. 2003 May;25(10):827-31. doi: 10.1023/a:1023568826461.
A phytase gene (appA) from Escherichia coli was cloned into Streptomyces lividans and expressed as an extracellular protein which was then compared with the same enzyme expressed in Pichia pastoris. The phytase expressed in S. lividans was not glycosylated and had a molecular mass of 45 kDa. Compared with the glycosylated phytase expressed in P. pastoris, this non-glycosylated phytase was 25-50% less active (p < 0.05) at pH 2 to 3.5 or at 45 and 55 degrees C, but 50% more active (p < 0.05) at 75 degrees C. The thermo-tolerance of the non-glycosylated phytase was 26 and 48% higher (p < 0.05) than that of the glycosylated phytase at 45 and 55 degrees C, but was 80 and 94% lower (p < 0.05) at 65 degrees and 75 degrees C, respectively.
将来自大肠杆菌的植酸酶基因（appA）克隆到淡紫链霉菌中，并表达为一种胞外蛋白，然后将其与在毕赤酵母中表达的同一种酶进行比较。在淡紫链霉菌中表达的植酸酶未进行糖基化，分子量为45 kDa。与在毕赤酵母中表达的糖基化植酸酶相比，这种非糖基化植酸酶在pH 2至3.5或45和55℃时活性低25 - 50%（p < 0.05），但在75℃时活性高50%（p < 0.05）。在45和55℃时，非糖基化植酸酶的耐热性比糖基化植酸酶分别高26%和48%（p < 0.05），但在65℃和75℃时分别低80%和94%（p < 0.05）。
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