Chang Chun-Sheng, Hsu Chin-Shuo
Department of Biotechnology, Southern Taiwan University of Technology, Tainan Hsien, Taiwan 710.
Biotechnol Lett. 2003 Mar;25(5):413-6. doi: 10.1023/a:1022948009889.
A lipase-catalyzed, enantioselective esterification process in organic solvents was developed for the synthesis of (S)-naproxen hydroxyalkyl ester. With the selection of lipase (Candida rugosa lipase) and reaction medium (isooctane and cyclohexane), a high enantiomeric ratio of > 100 for the enzyme was obtained. 1,4-Butanediol was the best acyl acceptor. The carbon chain length of the alcohol had a major effect on the enzyme activity and enantioselectivity of lipase-catalyzed esterification.
开发了一种在有机溶剂中由脂肪酶催化的对映选择性酯化方法，用于合成(S)-萘普生羟烷基酯。通过选择脂肪酶（皱褶假丝酵母脂肪酶）和反应介质（异辛烷和环己烷），该酶获得了大于100的高对映体比例。1,4-丁二醇是最佳的酰基受体。醇的碳链长度对脂肪酶催化酯化的酶活性和对映选择性有主要影响。
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