检测水中粪肠球菌的实时聚合酶链反应方法
Real-time PCR method to detect Enterococcus faecalis in water.
作者信息
Santo Domingo Jorge W, Siefring Shawn C, Haugland Richard A
机构信息
U.S. Environmental Protection Agency, National Exposure Research Laboratory, Microbial Exposure Research Branch, 26 W Martin Luther King Dr, Cincinnati, OH 45268, USA.
出版信息
Biotechnol Lett. 2003 Feb;25(3):261-5. doi: 10.1023/a:1022303118122.
A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.
开发了一种16S rDNA实时PCR方法来检测水样中的粪肠球菌。细胞检测的动态范围跨越五个对数级，检测限确定为6 cfu/反应。该检测方法能够检测添加到供水系统模拟器生物膜和淡水样本中的粪肠球菌细胞。无需核酸提取，可在不到3小时内检测到粪肠球菌细胞。
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