表达性基因组杂交：细胞遗传学水平的基因表达谱分析。
Expressive genomic hybridisation: gene expression profiling at the cytogenetic level.
作者信息
Al-Mulla F, Al-Maghrebi M, Varadharaj G
机构信息
Department of Pathology, Molecular Pathology Unit, Kuwait University, Faculty of Medicine, PO Box 24923, Safat, Kuwait 13110.
出版信息
Mol Pathol. 2003 Aug;56(4):210-7. doi: 10.1136/mp.56.4.210.
AIMS
To describe a cytogenetic technique suitable for the rapid assessment of global gene expression that is based on comparative genomic hybridisation (CGH), and to use it to understand the relation between genetic amplifications and gene expression.
METHODS
Whereas traditional CGH uses DNA as test and reference in hybridisations, expressive genomic hybridisation (EGH) uses globally amplified mRNA as test and normal DNA as reference. EGH is a rapid and powerful tool for localising and studying global gene expression profiles and correlating them with loci of genetic amplifications using traditional CGH.
RESULTS
EGH was used to correlate genetic amplifications detected by CGH with the expression profile of two independent cell lines-Colo320 and T47D. Although many amplifications resulted in overexpression, other amplifications were partially or completely silenced at the cytogenetic level.
CONCLUSION
This technique will assist in the analysis of overexpressed genes within amplicons and could resolve a controversial issue in cancer cytogenetics; namely, the relation between genetic amplifications and overexpression.
目的
描述一种基于比较基因组杂交（CGH）的适用于快速评估整体基因表达的细胞遗传学技术，并利用该技术了解基因扩增与基因表达之间的关系。
方法
传统CGH在杂交中使用DNA作为检测样本和参照样本，而表达性基因组杂交（EGH）则使用整体扩增的mRNA作为检测样本，正常DNA作为参照样本。EGH是一种快速且强大的工具，可用于定位和研究整体基因表达谱，并使用传统CGH将其与基因扩增位点相关联。
结果
EGH用于将CGH检测到的基因扩增与两种独立细胞系——Colo320和T47D的表达谱相关联。尽管许多扩增导致基因过表达，但其他扩增在细胞遗传学水平上部分或完全沉默。
结论
该技术将有助于分析扩增子内的过表达基因，并可能解决癌症细胞遗传学中的一个有争议的问题，即基因扩增与过表达之间的关系。
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