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本文引用的文献

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Impact of DNA amplification on gene expression patterns in breast cancer.DNA扩增对乳腺癌基因表达模式的影响。
Cancer Res. 2002 Nov 1;62(21):6240-5.
2
Amplification and expression of genes from the 17q11 approximately q12 amplicon in breast cancer cells.乳腺癌细胞中17q11至约q12扩增子基因的扩增与表达
Cancer Genet Cytogenet. 2002 Jul 1;136(1):43-7. doi: 10.1016/s0165-4608(01)00657-4.
3
Identification of amplified and expressed genes in breast cancer by comparative hybridization onto microarrays of randomly selected cDNA clones.通过与随机选择的cDNA克隆微阵列进行比较杂交来鉴定乳腺癌中扩增和表达的基因。
Genes Chromosomes Cancer. 2002 May;34(1):104-14. doi: 10.1002/gcc.10039.
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Silence of chromosomal amplifications in colon cancer.结肠癌中染色体扩增的沉默
Cancer Res. 2002 Feb 15;62(4):1134-8.
5
Gain of 5p15 detected by comparative genomic hybridization as an independent marker of poor prognosis in patients with esophageal squamous cell carcinoma.通过比较基因组杂交检测到的5号染色体短臂15区获得作为食管鳞状细胞癌患者预后不良的独立标志物。
Clin Cancer Res. 2002 Feb;8(2):526-33.
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Automated screening for genomic imbalances using matrix-based comparative genomic hybridization.使用基于矩阵的比较基因组杂交技术自动筛选基因组失衡情况。
Lab Invest. 2002 Jan;82(1):47-60. doi: 10.1038/labinvest.3780394.
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Assembly of microarrays for genome-wide measurement of DNA copy number.用于全基因组DNA拷贝数测量的微阵列组装。
Nat Genet. 2001 Nov;29(3):263-4. doi: 10.1038/ng754.
8
Comparative expressed sequence hybridization to chromosomes for tumor classification and identification of genomic regions of differential gene expression.用于肿瘤分类和差异基因表达基因组区域鉴定的染色体比较表达序列杂交。
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9197-202. doi: 10.1073/pnas.161272798.
9
Gain of 1q and loss of 9q21.3-q32 are associated with a less favorable prognosis in papillary thyroid carcinoma.
Genes Chromosomes Cancer. 2001 Sep;32(1):43-9. doi: 10.1002/gcc.1165.
10
Comprehensive copy number and gene expression profiling of the 17q23 amplicon in human breast cancer.人类乳腺癌中17q23扩增子的全面拷贝数和基因表达谱分析。
Proc Natl Acad Sci U S A. 2001 May 8;98(10):5711-6. doi: 10.1073/pnas.091582298. Epub 2001 May 1.

表达性基因组杂交：细胞遗传学水平的基因表达谱分析。

Expressive genomic hybridisation: gene expression profiling at the cytogenetic level.

作者信息

Al-Mulla F, Al-Maghrebi M, Varadharaj G

机构信息

Department of Pathology, Molecular Pathology Unit, Kuwait University, Faculty of Medicine, PO Box 24923, Safat, Kuwait 13110.

出版信息

Mol Pathol. 2003 Aug;56(4):210-7. doi: 10.1136/mp.56.4.210.

DOI:10.1136/mp.56.4.210
PMID:12890742
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1187323/
Abstract

AIMS

To describe a cytogenetic technique suitable for the rapid assessment of global gene expression that is based on comparative genomic hybridisation (CGH), and to use it to understand the relation between genetic amplifications and gene expression.

METHODS

Whereas traditional CGH uses DNA as test and reference in hybridisations, expressive genomic hybridisation (EGH) uses globally amplified mRNA as test and normal DNA as reference. EGH is a rapid and powerful tool for localising and studying global gene expression profiles and correlating them with loci of genetic amplifications using traditional CGH.

RESULTS

EGH was used to correlate genetic amplifications detected by CGH with the expression profile of two independent cell lines-Colo320 and T47D. Although many amplifications resulted in overexpression, other amplifications were partially or completely silenced at the cytogenetic level.

CONCLUSION

This technique will assist in the analysis of overexpressed genes within amplicons and could resolve a controversial issue in cancer cytogenetics; namely, the relation between genetic amplifications and overexpression.

摘要

目的

描述一种基于比较基因组杂交（CGH）的适用于快速评估整体基因表达的细胞遗传学技术，并利用该技术了解基因扩增与基因表达之间的关系。

方法

传统CGH在杂交中使用DNA作为检测样本和参照样本，而表达性基因组杂交（EGH）则使用整体扩增的mRNA作为检测样本，正常DNA作为参照样本。EGH是一种快速且强大的工具，可用于定位和研究整体基因表达谱，并使用传统CGH将其与基因扩增位点相关联。

结果

EGH用于将CGH检测到的基因扩增与两种独立细胞系——Colo320和T47D的表达谱相关联。尽管许多扩增导致基因过表达，但其他扩增在细胞遗传学水平上部分或完全沉默。

结论

该技术将有助于分析扩增子内的过表达基因，并可能解决癌症细胞遗传学中的一个有争议的问题，即基因扩增与过表达之间的关系。