用于临床样本中分枝杆菌直接检测的抗酸涂片优化。
Optimisation of acid fast smears for the direct detection of mycobacteria in clinical samples.
作者信息
Murray S J, Barrett A, Magee J G, Freeman R
机构信息
Regional Centre for Mycobacteriology, Public Health Laboratory, Institute of Pathology, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK.
出版信息
J Clin Pathol. 2003 Aug;56(8):613-5. doi: 10.1136/jcp.56.8.613.
AIMS
Despite its long history, the acid fast smear remains unstandardised. Technical variations in both the preparation of clinical material and subsequent staining mean that smear sensitivity relative to culture may vary from 50% to over 80%. This study assessed the sensitivity of acid fast microscopy at each of five stages of sample preparation and by both commonly used staining methods.
METHODS
Sputum samples thought for varying reasons to be highly likely to be culture positive were used to prepare a series of smears in which the effects of digestion (liquefaction), concentration (centrifugation), and decontamination (sodium hydroxide) could be assessed, together with a comparison of staining by the auramine/phenol and Ziehl-Neelsen techniques.
RESULTS
The most effective method for the demonstration of acid fast organisms in sputum was found to be an auramine phenol stain applied to a liquefied, concentrated sample and examined before the decontamination process.
CONCLUSIONS
The auramine phenol stain applied to a liquefied, concentrated sample and examined before the decontamination process is the most effective method for the demonstration of acid fast organisms in sputum.
目的
尽管抗酸涂片历史悠久，但仍未标准化。临床材料制备和后续染色的技术差异意味着涂片相对于培养的敏感性可能在50%至80%以上不等。本研究评估了样本制备五个阶段中每个阶段以及两种常用染色方法下抗酸显微镜检查的敏感性。
方法
使用因各种原因被认为极有可能培养阳性的痰液样本制备一系列涂片，评估消化（液化）、浓缩（离心）和去污（氢氧化钠）的效果，并比较金胺/苯酚染色法和齐-尼氏染色法。
结果
发现用于在痰液中显示抗酸菌的最有效方法是将金胺苯酚染色应用于液化、浓缩的样本，并在去污过程之前进行检查。
结论
将金胺苯酚染色应用于液化、浓缩的样本，并在去污过程之前进行检查是在痰液中显示抗酸菌的最有效方法。
Zotero 插件