Perret Camille, Pezet Roger, Tabacchi Raphael
Institut de Chimie, Université de Neuchâtel, Av. de Bellevaux 51, CH-2000 Neuchâtel, Switzerland.
Phytochem Anal. 2003 Jul-Aug;14(4):202-8. doi: 10.1002/pca.705.
Polymeric tannins, extracted from grape berries (Gamay variety), were fractionated according to their mean degree of polymerisation (mDP) on a styrene-divinylbenzene phase eluted with a gradient of methanol:chloroform. Increasing the percentage of methanol led to the solubilisation of higher molecular weight tannins. The mean mDP of each collected fraction was determined by acid-catalysed degradation in the presence of a nucleophilic reagent. The fractionation method produced a linear gradient of mDP varying between 1.84 and 19.34. The fractions were partially characterised by matrix assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). The spectra showed a complex mixture of proanthocyanidins and galloylated proanthocyanidins up to 4000 amu.
从葡萄浆果(佳美品种)中提取的聚合单宁,根据其平均聚合度(mDP),在以甲醇:氯仿梯度洗脱的苯乙烯 - 二乙烯基苯柱上进行分级分离。增加甲醇的百分比会使更高分子量的单宁溶解。每个收集级分的平均mDP通过在亲核试剂存在下的酸催化降解来测定。分级分离方法产生了mDP在1.84至19.34之间变化的线性梯度。这些级分通过基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF/MS)进行了部分表征。光谱显示了高达4000 amu的原花青素和没食子酰化原花青素的复杂混合物。