Liao Quan, Zhao Yu-pei, Kleeff Jorg, Xiao Yi, Zimmermann Arthur, Buchler Markus W, Friess Helmut
Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Wai Ke Za Zhi. 2003 May;41(5):328-31.
To study cartilage oligomeric matrix protein (COMP) mRNA and protein expression in normal pancreas, chronic pancreatitis (CP), and pancreatic cancer tissues.
Tissues from 15 cases of normal pancreas, 14 cases of chronic pancreatitis and 14 cases of pancreatic cancer were analyzed by Northern blot, Western blot, in situ hybridization and immunohistochemistry.
COMP mRNA signals and immunoreactivity were strongly present in the cytoplasm of degenerating acinar cells in CP tissues as well as in CP-like lesions in pancreatic cancer tissues. In contrast, COMP expression was weak to absent in the cytoplasm of cancer cells in pancreatic cancer tissues, and in ductal cells and islet cells in normal pancreatic tissues.
COMP is preferentially expressed in degenerating acinar cells in CP and in CP-like areas in pancreatic cancer, suggesting a potential role of this molecular in acinar cell dysfunction in CP.
研究软骨寡聚基质蛋白（COMP）mRNA和蛋白在正常胰腺、慢性胰腺炎（CP）及胰腺癌组织中的表达情况。
采用Northern印迹法、Western印迹法、原位杂交及免疫组化法对15例正常胰腺组织、14例慢性胰腺炎组织及14例胰腺癌组织进行分析。
COMP mRNA信号及免疫反应性在CP组织中退变腺泡细胞的细胞质以及胰腺癌组织中CP样病变中均强烈存在。相比之下，COMP在胰腺癌组织中癌细胞的细胞质、正常胰腺组织的导管细胞及胰岛细胞中表达较弱或无表达。
COMP在CP退变腺泡细胞及胰腺癌中CP样区域优先表达，提示该分子在CP腺泡细胞功能障碍中可能发挥作用。
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