[数字摄影后照法在白内障研究中对晶状体的应用]

Li Jian-jun, Xu Liang, Sun Bao-chen, Chen Ying-jie, Ma Ke

Beijing Institute of Ophthalmology, Beijing Tong Ren Ophthalomic Center, Capital Medical Science University, Beijing 100730, China.

Zhonghua Yan Ke Za Zhi. 2003 May;39(5):278-82.

OBJECTIVE

To evaluate the reliability of digital photography with retroillumination for lens as a tool to assess the degree of lens opacity, and to study the morphological features of some lens diseases using the digital images.

METHODS

The cataract screener (Neitz CT-S) was connected to a computer capable of acquiring digital images of lens. The digital lens retroillumination and slit lamp images were collected in 64 eyes. Three independent separate examiners took three sets of different CT-S digital photographs in four cataract subjects. The area of lens opacification in the images was measured and analyzed using threshold adjustment in PhotoShop software and a customized program in Matlab software.

RESULTS

The distinct digital images of lens cortical opacity, posterior subcapsular opacity and other lens diseases were obtained with CT-S computer digital photography, which images were much better than slit lamp sectional digital images. The sensitivity and specificity of measuring lens opacification with CT-S digital images were 90% and 94% respectively. However, the digital lens retroillumination images were not as sensitive as the slit lamp images for nuclear cataracts. The variation coefficients of lens opacity area in the three sets of CT-S digital images in the same subject, which were taken by the independent examiners, were from 2.23% to 10.86%. The cortical opacification in CT-S digital images was linear, cuniform or clustered aggregate shadow configuration. The posterior subcapsular opacification was discal vacuolar or granular appearance.

CONCLUSIONS

The reproducibility of the CT-S digital images taken by the independent examiners is excellent. This technique can objectively show cortical or posterior subcapsular opacities, but nuclear cataracts.

目的

评估采用后照法的数字摄影用于晶状体评估晶状体混浊程度的可靠性，并利用数字图像研究某些晶状体疾病的形态学特征。

方法

将白内障筛查仪（Neitz CT-S）连接到能够采集晶状体数字图像的计算机上。对64只眼采集数字晶状体后照法和裂隙灯图像。三名独立的检查者对四名白内障患者拍摄了三组不同的CT-S数字照片。使用Photoshop软件中的阈值调整和Matlab软件中的定制程序测量并分析图像中晶状体混浊的面积。

结果

通过CT-S计算机数字摄影获得了晶状体皮质混浊、后囊下混浊及其他晶状体疾病的清晰数字图像，这些图像比裂隙灯断层数字图像要好得多。用CT-S数字图像测量晶状体混浊的敏感性和特异性分别为90%和94%。然而，对于核性白内障，数字晶状体后照法图像不如裂隙灯图像敏感。由独立检查者拍摄的同一受试者的三组CT-S数字图像中晶状体混浊面积的变异系数为2.23%至10.86%。CT-S数字图像中的皮质混浊呈线性、楔形或簇状聚集阴影形态。后囊下混浊呈盘状空泡或颗粒状外观。