染色质免疫沉淀：一种研究组蛋白乙酰化和转录因子结合的工具。

Chromatin immunoprecipitation: a tool for studying histone acetylation and transcription factor binding.

作者信息

Spencer Virginia A, Sun Jian-Min, Li Lin, Davie James R

机构信息

Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0V9.

出版信息

Methods. 2003 Sep;31(1):67-75. doi: 10.1016/s1046-2023(03)00089-6.

DOI:10.1016/s1046-2023(03)00089-6

PMID:12893175

Abstract

The function of a protein in gene expression can often be explained, in part, by the location of that protein along a specific gene sequence. In recent years, the chromatin immunoprecipitation (ChIP) assay has been developed to study the association of proteins located within 2 A of DNA such as transcription factors and modified histones. Numerous important findings have been published using the ChIP assay and many questions about transcription have been answered. In this article, we present the ChIP assay currently used in our lab and discuss the various ways to optimize this assay for one's own use.

摘要

蛋白质在基因表达中的功能通常可以部分地通过该蛋白质沿着特定基因序列的位置来解释。近年来，染色质免疫沉淀（ChIP）分析技术已被开发出来，用于研究位于DNA 2A范围内的蛋白质（如转录因子和修饰的组蛋白）之间的关联。使用ChIP分析技术已经发表了许多重要的研究结果，并且许多关于转录的问题也得到了解答。在本文中，我们介绍了目前我们实验室使用的ChIP分析技术，并讨论了针对个人用途优化该分析技术的各种方法。