通过酵母双杂交方法检测C17orf25与ADP核糖焦磷酸酶NUDT9的相互作用。
Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9 detected via yeast two-hybrid method.
作者信息
Zhang Hui-Tang, Yan Zhi-Qiang, Hu Xiao-Bo, Yang Sheng-Li, Gong Yi
机构信息
Research Center of Biotechnology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233, China.
出版信息
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Aug;35(8):747-51.
The gene C17orf25 was isolated from the liver by RACE PCR. nudt9 gene was screened by yeast two-hybrid method in MatchMaker human HeLa cDNA library. NUDT9 is an enzyme that has pyrophosphatase activity with ADP-ribose as its substrate. Fusion expression of C17orf25 and GFP and computer analysis showed that C17orf25 was probably located in mitochondria. Furthermore, C17orf25 may suppress the cell growth by interaction with NUDT9.
通过RACE PCR从肝脏中分离出基因C17orf25。在MatchMaker人类HeLa cDNA文库中采用酵母双杂交法筛选nudt9基因。NUDT9是一种以ADP-核糖为底物具有焦磷酸酶活性的酶。C17orf25与绿色荧光蛋白（GFP）的融合表达及计算机分析表明C17orf25可能定位于线粒体。此外，C17orf25可能通过与NUDT9相互作用来抑制细胞生长。
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