Wang Rong, Hong Guo-Fan, Han Bin
National Center for Gene Research, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Aug;35(8):768-73.
A full-length Ty3-like retrotransposon, named RIRE10, was identified on the long arm of chromosome 4 in rice genome. The internal region between two LTRs had another open reading frame in the region upstream of gag-pol sequence. The transcripts from LTR region were detected by Northern blot hybridization and RT-PCR. To assess the activity of RIRE10 in rice genome, the copy number of its internal region and long terminal repeat (LTR) domain were determined by dot blot analyses. Nearly 900 solo-LTR of the RIRE10 retrotransposon exist in rice genome, apart from those LTRs that flank 65 intact RIRE retrotransposons. Based on the experimental results, the retrotransposition of RIRE10 was speculated to be influenced by two factors: transcriptional activity of LTR region and homologous recombination resulting in solo-LTR.
在水稻基因组的4号染色体长臂上鉴定出一个全长类Ty3反转录转座子，命名为RIRE10。两个长末端重复序列（LTR）之间的内部区域在gag-pol序列上游区域还有另一个开放阅读框。通过Northern杂交和逆转录聚合酶链反应（RT-PCR）检测到来自LTR区域的转录本。为了评估RIRE10在水稻基因组中的活性，通过斑点印迹分析确定其内部区域和长末端重复（LTR）结构域的拷贝数。除了位于65个完整RIRE反转录转座子两侧的那些LTR外，水稻基因组中存在近900个RIRE10反转录转座子的单拷贝LTR。基于实验结果，推测RIRE1０的反转录转座受两个因素影响：LTR区域的转录活性和导致单拷贝LTR的同源重组。
