San Martin S, Zorn T M T
Laboratory of Biology of Reproduction, Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, 05508-900 São Paulo, SP Brazil.
Cell Mol Biol (Noisy-le-grand). 2003 Jun;49(4):673-8.
In mice, embryo implantation induces profound changes in the endometrium. These changes include redifferentiation of endometrial fibroblasts and extensive remodeling of extracellular matrix components. We have previously shown that, during this process, there is an impressive increase in the thickness of collagen fibrils present in decidualised areas that surround the implantation site, while collagen fibrils in non-decidualised areas and in the interimplantation site remain thin. In vitro and in vivo experiments have identified small leucine rich proteoglycans (SLRPs) as regulators of collagen fibrillogenesis. In a previous study, we demonstrated a difference between the pre-implantation and the post-implantation expression and distribution of four SLRPs types in uterine tissues. The present study, utilising immunocytochemical electron microscopy, shows that biglycan is associated with the presence of thick collagen fibrils in decidualised regions of the endometrium and that decorin is associated exclusively with thin collagen fibrils in non-decidualised endometrial areas. These results strongly indicate that biglycan plays a role in collagen fibrillogenesis and probably participates in the determination of collagen fibril thickness in the mouse decidua.
在小鼠中，胚胎着床会引起子宫内膜的深刻变化。这些变化包括子宫内膜成纤维细胞的再分化以及细胞外基质成分的广泛重塑。我们之前已经表明，在此过程中，围绕着床部位的蜕膜化区域中存在的胶原纤维厚度显著增加，而未蜕膜化区域和着床间隙部位的胶原纤维仍然很细。体外和体内实验已确定富含亮氨酸的小分子蛋白聚糖（SLRPs）是胶原纤维形成的调节因子。在之前的一项研究中，我们证明了子宫组织中四种SLRPs类型在着床前和着床后的表达及分布存在差异。本研究利用免疫细胞化学电子显微镜显示，双糖链蛋白聚糖与子宫内膜蜕膜化区域中粗胶原纤维的存在有关，而核心蛋白聚糖仅与未蜕膜化子宫内膜区域中的细胶原纤维有关。这些结果有力地表明，双糖链蛋白聚糖在胶原纤维形成中起作用，并且可能参与了小鼠蜕膜中胶原纤维厚度的确定。
