Akata F, Tatman-Otkun M, Ozkan E, Tansel O, Otkun M, Tugrul M
Department of Clinical Bacteriology and Infectious Diseases, Medical Faculty of Trakya University, 22030 Edirne, Turkey.
New Microbiol. 2003 Jul;26(3):257-62.
The prevalence of extended-spectrum beta-lactamase (ESBL) production by 194 nosocomial isolates of Enterobacteriacea recovered from 1995 to 1999 was investigated. The ESBL production was determined by the double-disk synergy test and was confirmed by the E-test ESBL strip. Twenty-three isolates (21 Klebsiella pneumoniae, one Escherichia coli, one Providencia rettgeri) were found as ESBL-producers (11.8%). These isolates were also usually resistant to non-betalactam antibiotics. Most of them contained a beta-lactamase with a pI of 7.6. All the strains conjugally transferred their ESBLs to recipient E. coli. Contrary to others, ESBL-producing K. pneumoniae strains isolated in 1999 were resistant to ciprofloxacin, and had the identical plasmid profiles suggestive of an outbreak. Ciprofloxacin resistance in these strains could not be transferred. In conclusion, K. pneumoniae was the main ESBL-producing species among nosocomial isolates of Enterobacteriacae in our hospital.
对1995年至1999年从医院分离出的194株肠杆菌科细菌产生超广谱β-内酰胺酶（ESBL）的情况进行了调查。通过双纸片协同试验确定ESBL的产生，并通过E-test ESBL试纸条进行确认。发现23株分离菌（21株肺炎克雷伯菌、1株大肠埃希菌、1株雷氏普罗威登斯菌）为ESBL产生菌（11.8%）。这些分离菌通常也对非β-内酰胺类抗生素耐药。它们中的大多数含有pI为7.6的β-内酰胺酶。所有菌株都通过接合将其ESBL转移至受体大肠埃希菌。与其他菌株不同的是，1999年分离出的产ESBL肺炎克雷伯菌菌株对环丙沙星耐药，且具有相同的质粒图谱，提示有暴发流行。这些菌株中的环丙沙星耐药性无法转移。总之，肺炎克雷伯菌是我院医院分离肠杆菌科细菌中产ESBL的主要菌种。
