印度曼尼普尔邦人体感染异形吸虫（肺吸虫）的情况。
Occurrence of the lung fluke, Paragonimus heterotremus in Manipur, India.
作者信息
Singh T Shantikumar
机构信息
Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Sikkim, India.
出版信息
Chin Med Sci J. 2003 Mar;18(1):20-5.
OBJECTIVES
To determine the natural crustacean host, the Paragonimus species, and to investigate the potential host-parasite relationship between Manipur Paragonimus and some of the laboratory animals.
METHODS
The laboratory animals such as puppies, albino rats and Swiss mice were infected orally with metacercariae isolated from the fresh water crabs, Potamiscus manipurensis. The fecal specimens of the experimentally infected animals were examined microscopically for Paragonimus eggs at regular intervals. The animals were autopsied on days 35 to approximately 328 after infection and the isolated worms were flattened between glass slides and fixed in 70% alcohol. The worms were stained with carmine and mounted with Canada balsam for morphological studies. The eggs were collected in 5% formol saline solution for microscopy. The flukes were classified into 4 developmental stages.
RESULTS
A total of 11 worms, 5 mature, 5 immature and 1 pre-adult were recovered. The morphological features of the metacercariae, worms and eggs were similar to those of Paragonimus heterotremus.
CONCLUSION
Manipur is one of the rare areas in the world where Paragonimus heterotremus is prevalent and the puppies are ideal experimental animal host. This species may be one of the important causes of paragonimiasis in animals and humans in Manipur.
目的
确定自然甲壳类宿主、并殖吸虫种类，以及研究曼尼普尔并殖吸虫与一些实验动物之间潜在的宿主-寄生虫关系。
方法
将从淡水蟹曼尼普尔溪蟹分离出的尾蚴经口感染幼犬、白化大鼠和瑞士小鼠等实验动物。定期对实验感染动物的粪便标本进行显微镜检查，以查找并殖吸虫卵。在感染后35天至约328天对动物进行解剖，将分离出的虫体置于载玻片之间压平，并用70%酒精固定。虫体用洋红染色，并用加拿大树胶封固以进行形态学研究。将虫卵收集在5%甲醛盐溶液中用于显微镜检查。吸虫分为4个发育阶段。
结果
共回收11条虫体，其中5条成熟、5条未成熟和1条成虫前期。尾蚴、虫体和虫卵的形态特征与异形并殖吸虫相似。
结论
曼尼普尔是世界上异形并殖吸虫流行的罕见地区之一，幼犬是理想的实验动物宿主。该物种可能是曼尼普尔动物和人类并殖吸虫病的重要病因之一。
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