用基质辅助激光解吸电离离子淌度-飞行时间质谱分析血管紧张素II-乙酰胆碱非共价复合物。
Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS.
作者信息
Woods Amina S, Fuhrer Katrin, Gonin Marc, Egan Tom, Ugarov Michael, Gillig Kent J, Schultz J Albert
机构信息
NIDA IRP, NIH, Baltimore, MD 21224, USA.
出版信息
J Biomol Tech. 2003 Mar;14(1):1-8.
Abstract
Matrix-assisted laser desorption ionization-ion mobility-orthogonal time-of-flight mass spectrometry (MALDI-IM oTOF MS) is a new technique that allows laser desorbed ion to be preseparated on the basis of their shape prior to mas analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angio tensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.
摘要
基质辅助激光解吸电离-离子淌度-正交飞行时间质谱（MALDI-IM oTOF MS）是一种新技术，它能使激光解吸离子在质谱分析前根据其形状进行预分离。使用该仪器，我们验证了这样一个假设：向模型磷酸化肽血管紧张素II中添加季铵化合物（如乙酰胆碱）会通过两种方式提高其在MALDI检测中的效果。首先，乙酰胆碱-肽复合物比裸露的磷酸肽能更有效地电离。此外，离子淌度可根据复合物离子的电荷/体积将其与等压干扰物分离，否则等压干扰物会限制检测灵敏度。
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