用基质辅助激光解吸电离离子淌度-飞行时间质谱分析血管紧张素II-乙酰胆碱非共价复合物。
Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS.
作者信息
Woods Amina S, Fuhrer Katrin, Gonin Marc, Egan Tom, Ugarov Michael, Gillig Kent J, Schultz J Albert
机构信息
NIDA IRP, NIH, Baltimore, MD 21224, USA.
出版信息
J Biomol Tech. 2003 Mar;14(1):1-8.
Abstract
Matrix-assisted laser desorption ionization-ion mobility-orthogonal time-of-flight mass spectrometry (MALDI-IM oTOF MS) is a new technique that allows laser desorbed ion to be preseparated on the basis of their shape prior to mas analysis. Using this instrument, we tested the postulate that addition of a quaternary ammonium compound such as acetylcholine to the model phosphorylated peptide angio tensin II would enhance its detection by MALDI in two ways. First of all, the acetylcholine-peptide complex could ionize more efficiently than the bare phosphopeptide. Furthermore the ion mobility could separate the complex ion on the basis of its charge/volume from isobaric interferences, which would otherwise limit detection sensitivity.
摘要
基质辅助激光解吸电离-离子淌度-正交飞行时间质谱（MALDI-IM oTOF MS）是一种新技术，它能使激光解吸离子在质谱分析前根据其形状进行预分离。使用该仪器，我们验证了这样一个假设：向模型磷酸化肽血管紧张素II中添加季铵化合物（如乙酰胆碱）会通过两种方式提高其在MALDI检测中的效果。首先，乙酰胆碱-肽复合物比裸露的磷酸肽能更有效地电离。此外，离子淌度可根据复合物离子的电荷/体积将其与等压干扰物分离，否则等压干扰物会限制检测灵敏度。
相似文献
1
Angiotensin II-acetylcholine noncovalent complexes analyzed with MALDI-ion mobility-TOF MS.
J Biomol Tech. 2003 Mar;14(1):1-8.
2
Characterization and sequence identification of angiotensin II by a novel method involving ultra-fast liquid chromatography assay coupled with matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight five tandem mass spectrometry analysis.
Eur J Mass Spectrom (Chichester). 2010;16(6):663-71. doi: 10.1255/ejms.1099.
3
Analysis of noncovalent chitinase-chito-oligosaccharide complexes by infrared-matrix assisted laser desorption ionization and nanoelectrospray ionization mass spectrometry.
Anal Chem. 2011 Jun 1;83(11):4030-6. doi: 10.1021/ac1031308. Epub 2011 May 5.
4
Nonradioactive phosphopeptide assay by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: application to calcium/calmodulin-dependent protein kinase II.
Anal Biochem. 1998 Jul 1;260(2):188-94. doi: 10.1006/abio.1998.2691.
5
Lipid/peptide/nucleotide separation with MALDI-ion mobility-TOF MS.
Anal Chem. 2004 Apr 15;76(8):2187-95. doi: 10.1021/ac035376k.
6
A study of peptide-peptide interactions using MALDI ion mobility o-TOF and ESI mass spectrometry.
J Am Soc Mass Spectrom. 2002 Feb;13(2):166-9. doi: 10.1016/S1044-0305(01)00348-8.
7
Distinguishing between phosphorylated and nonphosphorylated peptides with ion mobility-mass spectrometry.
J Proteome Res. 2002 Jul-Aug;1(4):303-6. doi: 10.1021/pr025516r.
8
Cellular membrane phospholipids act as a depository for quaternary amine containing drugs thus competing with the acetylcholine/nicotinic receptor.
J Proteome Res. 2012 Jun 1;11(6):3382-9. doi: 10.1021/pr300184g. Epub 2012 Apr 30.
9
Sub-femtomole peptide detection in ion mobility-time-of-flight mass spectrometry measurements.
J Proteome Res. 2003 Jul-Aug;2(4):427-30. doi: 10.1021/pr034004p.
10
Black phosphorus-assisted laser desorption ionization mass spectrometry for the determination of low-molecular-weight compounds in biofluids.
Anal Bioanal Chem. 2016 Sep;408(22):6223-33. doi: 10.1007/s00216-016-9737-z. Epub 2016 Jul 6.
引用本文的文献
1
MALDI/post ionization-ion mobility mass spectrometry of noncovalent complexes of dopamine receptors' epitopes.
J Proteome Res. 2013 Apr 5;12(4):1668-77. doi: 10.1021/pr301004w. Epub 2013 Mar 25.
2
Cellular membrane phospholipids act as a depository for quaternary amine containing drugs thus competing with the acetylcholine/nicotinic receptor.
J Proteome Res. 2012 Jun 1;11(6):3382-9. doi: 10.1021/pr300184g. Epub 2012 Apr 30.
3
A study of phospholipids by ion mobility TOFMS.
J Am Soc Mass Spectrom. 2008 Nov;19(11):1655-62. doi: 10.1016/j.jasms.2008.07.005. Epub 2008 Jul 16.
4
Amazing stability of phosphate-quaternary amine interactions.
J Proteome Res. 2008 Aug;7(8):3423-7. doi: 10.1021/pr8001595. Epub 2008 Jun 26.
5
Orthogonal extraction ion mobility spectrometry.
J Am Soc Mass Spectrom. 2006 Apr;17(4):500-507. doi: 10.1016/j.jasms.2005.12.009. Epub 2006 Feb 28.
6
Using amino acids for probing structural information of cytochrome c by electrospray ionization mass spectrometry.
J Am Soc Mass Spectrom. 2004 Nov;15(11):1612-5. doi: 10.1016/j.jasms.2004.07.017.
本文引用的文献
1
Electrospray ionization high-resolution ion mobility spectrometry-mass spectrometry.
Anal Chem. 1998 Dec 1;70(23):4929-38. doi: 10.1021/ac980414z.
2
Distinguishing between phosphorylated and nonphosphorylated peptides with ion mobility-mass spectrometry.
J Proteome Res. 2002 Jul-Aug;1(4):303-6. doi: 10.1021/pr025516r.
3
Observation of conserved solution-phase secondary structure in gas-phase tryptic peptides.
J Am Chem Soc. 2002 Apr 24;124(16):4214-5. doi: 10.1021/ja0178113.
4
Fragmentation of phosphopeptides by atmospheric pressure MALDI and ESI/Ion trap mass spectrometry.
J Am Soc Mass Spectrom. 2002 Mar;13(3):274-83. doi: 10.1016/S1044-0305(01)00361-0.
5
A study of peptide-peptide interactions using MALDI ion mobility o-TOF and ESI mass spectrometry.
J Am Soc Mass Spectrom. 2002 Feb;13(2):166-9. doi: 10.1016/S1044-0305(01)00348-8.
6
Helix formation in unsolvated peptides: side chain entropy is not the determining factor.
J Am Chem Soc. 2001 Aug 15;123(32):7907-8. doi: 10.1021/ja010891q.
7
Gas-phase separations of complex tryptic peptide mixtures.
Fresenius J Anal Chem. 2001 Feb;369(3-4):234-45. doi: 10.1007/s002160000669.
8
Peptide sequence information derived by pronase digestion and ammonium sulfate in-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
J Am Soc Mass Spectrom. 2000 Nov;11(11):1000-8. doi: 10.1016/S1044-0305(00)00170-7.
9
Coupling high-pressure MALDI with ion mobility/orthogonal time-of-flight mass spectrometry.
Anal Chem. 2000 Sep 1;72(17):3965-71. doi: 10.1021/ac0005619.
10
Phosphorylation of simian cytomegalovirus assembly protein precursor (pAPNG.5) and proteinase precursor (pAPNG1): multiple attachment sites identified, including two adjacent serines in a casein kinase II consensus sequence.
J Virol. 1999 Nov;73(11):9053-62. doi: 10.1128/JVI.73.11.9053-9062.1999.
Zotero 插件