RNA乐高积木：通过环-环相互作用实现依赖镁的RNA构建模块组装。

RNA LEGO: magnesium-dependent assembly of RNA building blocks through loop-loop interactions.

作者信息

Horiya Satoru, Li Xianglan, Kawai Gota, Saito Ryota, Katoh Akira, Kobayashi Koh, Harada Kazuo

机构信息

Department of Life Science, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan.

出版信息

Nucleic Acids Res Suppl. 2002(2):41-2. doi: 10.1093/nass/2.1.41.

DOI:10.1093/nass/2.1.41

PMID:12903095

Abstract

We describe the construction of nano-molecular assemblies using RNA building blocks the human immunodeficiency virus type 1 (HIV-1) dimerization initiation site (DIS) RNA, that forms stable base pairing through a magnesium-dependent loop-loop interaction ("kissing"). RNA building blocks containing two DIS or DIS-like hairpins connected by a two nucleotide linker self-assembled to form specific structures as observed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Furthermore, observation of "real time" formation of the molecular assemblies by circular dichroism (CD) spectroscopy was attempted.

摘要

我们描述了使用RNA构建模块构建纳米分子组装体的过程，该构建模块为人免疫缺陷病毒1型（HIV-1）二聚化起始位点（DIS）RNA，它通过镁依赖性环-环相互作用（“亲吻”）形成稳定的碱基配对。含有两个由两个核苷酸接头连接的DIS或类DIS发夹的RNA构建模块自组装形成特定结构，这通过非变性聚丙烯酰胺凝胶电泳（PAGE）观察到。此外，还尝试通过圆二色性（CD）光谱法“实时”观察分子组装体的形成。