[胞嘧啶脱氨酶/5-氟胞嘧啶对人胰腺癌细胞生长的抑制作用]
[Cytosine diaminase/5-flurocytosine mediated inhibiting effect on the growth of human pancreatic carcinoma cells].
作者信息
Zhang L, Liu T, Cui Q, Gao J
机构信息
Department of Pathology, PUMC Hospital, CAMS, PUMC, Beijing 100730, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2000 Apr;22(2):139-43.
OBJECTIVE
To study the inhibition effect of E. coli cytosine diaminase (CD)/5-flurocytosine (5-FC) system on the growth of human pancreatic carcinoma cell line cells.
METHODS
Recombinant retroviral vector expressing CD genes was transduced into pancreatic carcinoma cell line cells. In vitro and in vivo prodrug sensitivity assays were carried out, including: (1) detection of growth and colony forming inhibition rate of transduced cells in the presence of 5-FC; (2) observation of bystander effect by MTT method; (3) observation of the growth inhibition effect of 5-FC on transduced cell xenografts in nude mice.
RESULTS
The growth and colony forming inhibition rate of the transduced cells in the presence of 5-FC were 80.0% and 79.0%, which were obviously higher than 4.8% and 5.0% (P < 0.01) of nontransduced cells. Mixed cells containing only 10% of transduced cells showed 50.7% reduction of proliferation (bystander effect). The growth of transduced cells xenografts could be totally regressed by 5-FC.
CONCLUSIONS
CD/5-FC system is a potential gene therapy strategy for pancreatic carcinoma.
目的
研究大肠杆菌胞嘧啶脱氨酶（CD）/5-氟胞嘧啶（5-FC）系统对人胰腺癌细胞系细胞生长的抑制作用。
方法
将表达CD基因的重组逆转录病毒载体转导至胰腺癌细胞系细胞中。进行体外和体内前药敏感性试验，包括：（1）检测转导细胞在5-FC存在下的生长和集落形成抑制率；（2）用MTT法观察旁观者效应；（3）观察5-FC对裸鼠中转导细胞异种移植物的生长抑制作用。
结果
转导细胞在5-FC存在下的生长和集落形成抑制率分别为80.0%和79.0%，明显高于未转导细胞的4.8%和5.0%（P<0.01）。仅含10%转导细胞的混合细胞增殖减少50.7%（旁观者效应）。5-FC可使转导细胞异种移植物的生长完全消退。
结论
CD/5-FC系统是一种有潜力的胰腺癌基因治疗策略。
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