Lu Fachuang, Ralph John
U.S. Dairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USA.
Plant J. 2003 Aug;35(4):535-44. doi: 10.1046/j.1365-313x.2003.01817.x.
Two solvent systems for fully dissolving, and optionally derivatizing, finely ground plant cell wall material at room temperature are described: dimethylsulfoxide (DMSO) and tetrabutylammonium fluoride (TBAF) or N-methylimidazole (NMI). In situ acetylation produces acetylated cell walls (Ac-CWs) that are fully soluble in chloroform. Lignin structures tested remain fully intact. The dispersion of 13C-1H correlations afforded by two-dimensional (2D) nuclear magnetic resonance (NMR) experiments reveals the major lignin units, allowing the whole lignin fraction to be analyzed by high-resolution solution-state NMR methods for the first time. Non-degradative cell wall dissolution offers the potential to analyze polysaccharide components, and improve current cell wall analytical methods by using standard homogeneous solution-state chemistry.
二甲基亚砜（DMSO）和四丁基氟化铵（TBAF）或N-甲基咪唑（NMI）。原位乙酰化可产生完全可溶于氯仿的乙酰化细胞壁（Ac-CW）。所测试的木质素结构保持完全完整。二维（2D）核磁共振（NMR）实验提供的¹³C-¹H相关性分散揭示了主要的木质素单元，首次使得整个木质素部分能够通过高分辨率溶液态NMR方法进行分析。非降解性细胞壁溶解为分析多糖成分提供了潜力，并通过使用标准的均相溶液态化学方法改进了当前的细胞壁分析方法。
