[胎儿骨髓来源的Flk1+ CD34-细胞的血管母细胞特征]
[Hemangioblastic characteristics of fetal bone marrow-derived Flk1+ CD34- cells].
作者信息
Guo Hong, Zhao Zhi-gang, Liu Jie-wen, Chen Hui-shu, Zhao Chun-hua
机构信息
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS, PUMC, Tianjin 300020, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Dec;24(6):606-10.
OBJECTIVE
To investigate whether fetal bone marrow stromal cells have hemangioblastic characteristics.
METHODS
Human fetal bone marrow stromal cells (hfMSCs) were isolated and cultured. Immunophenotypes of hfMSCs were tested by FACS. hfMSCs seeded in the matrigel were induced with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in vitro. Vascularization and hematopoiesis were detected with immunohistochemistry and electron microscope.
RESULTS
The typical properties of this CD34- stromal cell population were that 99% cells expressed Flk1 (vascular endothelial cell growth factor receptor 2) and tube structure was formed. In the process of induction, hfMSCs could give rise to CD34+ round cells.
CONCLUSIONS
We have demonstrated that fetal bone marrow stroma-derived Flk1+ CD34- cells could differentiate into vascular endothelial cells and hematopoietic cells, indicating that fetal bone marrow stroma-derived Flk1+ CD34- cells have hemangioblastic characteristics.
目的
研究胎儿骨髓基质细胞是否具有成血管细胞特性。
方法
分离并培养人胎儿骨髓基质细胞（hfMSCs）。通过流式细胞术检测hfMSCs的免疫表型。将接种于基质胶中的hfMSCs在体外使用血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）进行诱导。采用免疫组织化学和电子显微镜检测血管生成和造血情况。
结果
该CD34-基质细胞群体的典型特性为99%的细胞表达Flk1（血管内皮细胞生长因子受体2）并形成管状结构。在诱导过程中，hfMSCs可产生CD34+圆形细胞。
结论
我们已证明胎儿骨髓基质来源的Flk1+ CD34-细胞可分化为血管内皮细胞和造血细胞，表明胎儿骨髓基质来源的Flk1+ CD34-细胞具有成血管细胞特性。
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