大鼠肝脏再生增强因子基因组DNA序列的克隆与分析
Cloning and analysis of the genomic DNA sequence of augmenter of liver regeneration from rat.
作者信息
Dong Jing, Cheng Jun, Wang Qinhuan, Shi Shuangshuang, Wang Gang, Si Chongwen
机构信息
Gene Therapy Research Center, Institute of Infectious Diseases, the 302 Hospital of PLA, Beijing 100039.
出版信息
Chin Med Sci J. 2002 Jun;17(2):63-7.
OBJECTIVE
To search for genomic DNA sequence of the augmenter of liver regeneration (ALR) of rat.
METHODS
Polymerase chain reaction (PCR) with specific primers was used to amplify the sequence from the rat genome.
RESULTS
A piece of genomic DNA sequence and a piece of pseudogene of rat ALR were identified. The lengths of the gene and pseudogene are 1508 bp and 442 bp, respectively. The ALR gene of rat includes 3 exons and 2 introns. The 442 bp DNA sequence may represent a pseudogene or a ALR-related peptide. Predicted amino acid sequence analysis showed that there were 14 different amino acid residues between the gene and pseudogene. ALR-related peptide is 84 amino acid residues in length and relates closely to ALR protein.
CONCLUSION
There might be a multigene family of ALR in rat.
目的
寻找大鼠肝脏再生增强因子（ALR）的基因组DNA序列。
方法
使用特异性引物通过聚合酶链反应（PCR）从大鼠基因组中扩增该序列。
结果
鉴定出一段大鼠ALR的基因组DNA序列和一段假基因。该基因和假基因的长度分别为1508 bp和442 bp。大鼠的ALR基因包括3个外显子和2个内含子。这442 bp的DNA序列可能代表一个假基因或一个ALR相关肽。预测的氨基酸序列分析表明，该基因和假基因之间有14个不同的氨基酸残基。ALR相关肽长度为84个氨基酸残基，与ALR蛋白密切相关。
结论
大鼠中可能存在ALR多基因家族。
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