Ohata Atsushi, Usami Makoto, Horiuchi Takashi, Nagasawa Koichi, Kinoshita Keiko
Faculty of Health Sciences, Kobe University School of Medicine, Kobe, Japan.
Artif Organs. 2003 Aug;27(8):728-35. doi: 10.1046/j.1525-1594.2003.07137.x.
To clarify the origin of (1-->3)-beta-D-glucan in blood products and assess the biological activity of filter extracts, we evaluated (1-->3)-beta-D-glucan extraction from depth filters used to process blood products and their in vitro effects on proinflammatory cytokine production from macrophages. Cellulose or nylon filters were analyzed for (1-->3)-beta-D-glucan using the Fungitec G test. To evaluate the biological activity of the filter extracts, Mono Mac 6 cells (a human macrophage cell line) were cultured with filter extracts with or without lipopolysaccharide, and tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1beta) concentrations in the culture media were measured. (1-->3)-beta-D-Glucan was released from seven cellulose filters but the nylon filter level was undetectable. Proinflammatory cytokine production ranged from 74.3% to 119.0% of the control for TNF-alpha and 81.2% to 115.9% for IL-1beta. TNF-alpha and IL-1beta levels were low without lipopolysaccharide. The data indicate that (1-->3)-beta-D-glucan in blood products is contaminated with the depth filters and that these filter extracts modulate proinflammatory cytokine production from macrophages.
为了阐明血液制品中(1→3)-β-D-葡聚糖的来源并评估滤器提取物的生物活性，我们评估了用于处理血液制品的深层滤器中(1→3)-β-D-葡聚糖的提取情况及其对巨噬细胞促炎细胞因子产生的体外影响。使用Fungitec G试验分析纤维素或尼龙滤器中的(1→3)-β-D-葡聚糖。为了评估滤器提取物的生物活性，将Mono Mac 6细胞（一种人巨噬细胞系）与有或无脂多糖的滤器提取物一起培养，并测量培养基中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的浓度。(1→3)-β-D-葡聚糖从七个纤维素滤器中释放出来，但尼龙滤器中的水平无法检测到。促炎细胞因子的产生范围为TNF-α的对照组的74.3%至119.0%，IL-1β的为81.2%至115.9%。无脂多糖时TNF-α和IL-1β水平较低。数据表明血液制品中的(1→3)-β-D-葡聚糖被深层滤器污染，并且这些滤器提取物调节巨噬细胞促炎细胞因子的产生。
