Koyama T, Omata Y, Saito A
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan.
Horm Metab Res. 2003 Jun;35(6):355-7. doi: 10.1055/s-2003-41356.
The purpose of this investigation was to clarify whether salivary cortisol secretion in dogs had a circadian rhythm. Saliva sampling during a 24-hour period was performed in 4 non-consecutive days. Eight adult beagle dogs (4 males and 4 females) were divided into 4 groups, and 2 dogs (1 male and 1 female) were used for each repetition. Saliva samples were taken at 1 h intervals from 9:00 a. m. to 9:00 a. m. of the following day. Salivary cortisol concentrations were determined using enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary cortisol, and the differences among salivary cortisol concentrations measured every hour were not demonstrated during a 24-hour period in dogs.
本研究的目的是阐明犬唾液皮质醇分泌是否具有昼夜节律。在4个非连续日的24小时内进行唾液采样。8只成年比格犬（4只雄性和4只雌性）分为4组，每次重复使用2只犬（1只雄性和1只雌性）。从上午9:00至次日上午9:00每隔1小时采集唾液样本。使用酶联免疫吸附测定法测定唾液皮质醇浓度。未检测到犬唾液皮质醇的昼夜节律，且在犬的24小时期间内每小时测量的唾液皮质醇浓度之间的差异未得到证实。
