Wang Zhao-wen, Peng Zhi-hai, Li Ke, Qiu Guo-qiang, Zhang Yi, Gu Wei
Department of General Surgery, Shanghai First People Hospital, Shanghai 200080, China.
Zhonghua Zhong Liu Za Zhi. 2003 Jul;25(4):356-8.
To investigate the relation between p16 gene expression and the carcinogenesis and progress of hepatitis B virus (HBV) related hepatocellular carcinoma (HCC).
In 35 specimens of HCC tissue and the adjacent liver tissue, the integration of HBV X gene was detected by polymerase chain reaction (PCR) and Southern blot. The point mutation of exon-1alpha, 2 and 3 of p16 gene were detected by PCR-single strand conformation polymorphism (SSCP). The expression of p16 mRNA and p16 protein was detected by RT-PCR and Western blot.
The integration of X gene correlated with the expression loss of p16 mRNA and p16 protein in HCC (P < 0.05). The expression loss rates of p16 protein in HCC and adjacent tissues were 62.9% (22/35) and 40.0% (14/35) with significant difference (P < 0.05). The expression loss of p16 protein in HCC correlated with the differentiation degrees of HCC and the infiltration of tumor cells (P < 0.05).
The integration of X gene correlates with the expression loss of p16 protein. The alteration of p16 gene, playing an important role in all stages of hepatocarcinogenesis, correlates with the progress and invasion of hepatocellular carcinoma.
探讨p16基因表达与乙型肝炎病毒（HBV）相关肝细胞癌（HCC）发生及进展的关系。
采用聚合酶链反应（PCR）及Southern印迹法检测35例HCC组织及其癌旁肝组织中HBV X基因的整合情况；采用PCR-单链构象多态性（SSCP）法检测p16基因第1α、2及3外显子的点突变情况；采用逆转录-聚合酶链反应（RT-PCR）及蛋白质免疫印迹法（Western blot）检测p16 mRNA及p16蛋白的表达情况。
HCC组织中X基因的整合与p16 mRNA及p16蛋白表达缺失相关（P<0.05）。HCC组织及癌旁组织中p16蛋白表达缺失率分别为62.9%（22/35）和40.0%（14/35），差异有统计学意义（P<0.05）。HCC组织中p16蛋白表达缺失与HCC的分化程度及肿瘤细胞浸润相关（P<0.05）。
X基因整合与p16蛋白表达缺失相关。p16基因改变在肝癌发生各阶段起重要作用，与肝细胞癌进展及侵袭相关。
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