Luo Feng-ming, Wang Zeng-li, Liu Xiao-jing, Liu Chun-tao, Zhang Xiao-hong, Wang Wen-zhi
Department of Respiratory Disease, Huaxi Hospital of Sichuan University, Chengdu 610041, China.
Zhonghua Nei Ke Za Zhi. 2003 Jul;42(7):466-9.
To investigate the mRNA expression of Clara cell secretory protein (CCSP) and the Clara cell number in airways of rat asthma remodel.
A rat asthma model was established by sensitization and challenge with ovalbumin (OA). The mRNA expression of CCSP in the lung tissue, the CCSP level in bronchial alveolar lavage fluid (BALF), the thickness of the airways and the number of Clara cells in bronchioles were determined by RT-PCR, image analysis system, dot immunoblotting and immunohistochemistry methods.
There was a progressive decline in CCSP mRNA expression in the lung tissue of rat asthma model group (0.53 +/- 0.07 and 0.49 +/- 0.03, respectively, after 1 week and 2 weeks of challenge) as compared to that of control group (0.67 +/- 0.04). The CCSP levels in BALF of asthma model group (47.00 +/- 6.58 and 45.95 +/- 3.20, respectively, after 1 week and 2 weeks of challenge) were significantly decreased than that of control group (63.08 +/- 2.84, P < 0.01). The ratio(%) of Clara cells was also reduced after challenge. The WAt/Pbm, WAi/Pbm, and WAm/Pbm were significantly increased 2 weeks after OA challenge and were negatively correlated with the level of CCSP and its mRNA expression.
The Clara cells and CCSP may participate in the pathogenesis of asthma in the rat asthma remodel.
研究克拉拉细胞分泌蛋白（CCSP）的mRNA表达及哮喘重塑大鼠气道中克拉拉细胞数量。
用卵清蛋白（OA）致敏和激发建立大鼠哮喘模型。采用逆转录聚合酶链反应（RT-PCR）、图像分析系统、斑点免疫印迹法和免疫组织化学方法，检测肺组织中CCSP的mRNA表达、支气管肺泡灌洗液（BALF）中CCSP水平、气道厚度及细支气管中克拉拉细胞数量。
与对照组（0.67±0.04）相比，哮喘模型组大鼠肺组织中CCSP mRNA表达呈进行性下降（激发1周和2周后分别为0.53±0.07和0.49±0.03）。哮喘模型组BALF中CCSP水平（激发1周和2周后分别为47.00±6.58和45.95±3.20）明显低于对照组（63.08±2.84，P<0.01）。激发后克拉拉细胞比例（%）也降低。OA激发2周后，WAt/Pbm、WAi/Pbm和WAm/Pbm显著增加，且与CCSP水平及其mRNA表达呈负相关。
在哮喘重塑大鼠中，克拉拉细胞和CCSP可能参与哮喘的发病机制。
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