Jiang Du-yin, Chen Bi, Jia Chi-yu, Tao Ke
Department of Burns, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China.
Zhonghua Shao Shang Za Zhi. 2003 Jun;19(3):155-8.
To investigate the difference of the antigenicity of xenogenic acellular dermal matrix (ADM) implants prepared by different methods.
The split-thickness skin sheet from swine was processed by trypsin and Triton X-100 to make xeno-ADM. Twenty-five Japanese white rabbits were divided into 5 groups, i.e. xeno-ADM(1) (conjugated with glutaraldehyde), xeno-ADM(2) (conjugated with network) and xeno-ADM(3) (no conjugation, as control), in which the ADMs were and xeno-ADM(4) (conjugated) and allo-ADM (no conjugated as control), in which the ADMs were embedded into the subcutaneous place of rabbit ear and back after that the rabbits were pre-sensitized by xeno-ADM soluble protein antigen injections. The titers of anti ADMs antibody in rabbit serum were monitored during 2 - 32 post-operative weeks and the histological changes of the embedded ADMs were observed grossly and microscopically.
The serum titers of anti-xeno-ADM in xeno-ADM(4) group was the highest. Whereas regardless of the sensitizing effects, the titers in all groups ranged as follows: xeno-ADM(3) > xeno-ADM(2) > xeno-ADM(1) (P < 0.05 - 0.01). About 40% serum samples in allo-ADM group exhibited positive anti-allo-ADM protein antibodies. Histologically, Evident and lasting inflammatory reaction could be found in the xeno-ADM grafting sites, which was much stronger than that in allo-ADM group. The degradation and absorption gradient of ADM was ranked as follow: xeno-ADM(3) > xeno-ADM(2) > xeno-ADM(4) > xeno-ADM(1) > Allo-ADM. Foreign body megalocytic reaction might evoke in the surrounding of conjugated ADM.
The immunogenicity in xeno-ADM was stronger than that in allo-ADM, which could induce the host to develop immune reaction restricted by IgG. Large sheets of degenerated ADM implants could lower down the antigen-antibody reaction and ameliorate the structural destroying and degeneration absorption of ADM induced by inflammatory immune reaction.
探讨不同方法制备的异种脱细胞真皮基质（ADM）植入物的抗原性差异。
用胰蛋白酶和 Triton X - 100处理猪的断层皮片制成异种ADM。将25只日本白兔分为5组，即异种ADM(1)（与戊二醛结合）、异种ADM(2)（与网状物结合）和异种ADM(3)（未结合，作为对照），其中将ADM植入兔耳和背部皮下，之后用异种ADM可溶性蛋白抗原注射对兔进行预致敏。在术后2 - 32周监测兔血清中抗ADM抗体的滴度，并大体和显微镜下观察植入ADM的组织学变化。
异种ADM(4)组抗异种ADM的血清滴度最高。而无论致敏效果如何，各组滴度排序如下：异种ADM(3)＞异种ADM(2)＞异种ADM(1)（P＜0.05 - 0.01）。同种异体ADM组约40%的血清样本显示抗同种异体ADM蛋白抗体呈阳性。组织学上，在异种ADM移植部位可发现明显且持久的炎症反应，比同种异体ADM组要强得多。ADM的降解和吸收梯度排序如下：异种ADM(3)＞异种ADM(2)＞异种ADM(4)＞异种ADM(1)＞同种异体ADM。结合的ADM周围可能引发异物巨细胞反应。
异种ADM的免疫原性强于同种异体ADM，可诱导宿主产生受IgG限制的免疫反应。大片变性的ADM植入物可降低抗原 - 抗体反应，改善炎症免疫反应诱导的ADM结构破坏和变性吸收。
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