[L-精氨酸和NG-硝基-L-精氨酸甲酯对脂多糖和脂磷壁酸诱导的大鼠肝损伤的影响]

[The effects of L-arginine and NG-nitro-L-arginine methylester on lipopolysaccharide and lipoteichoic acid induced liver injury in the rat].

作者信息

Engin Atilla, Kurukahvecioğlu Osman, Bukan Neslihan, Dursun Ayşe

机构信息

Gazi Universitesi, Tip Fakültesi, Genel Cerraihi Anabilim Dali, Beşevler, Ankara, Turkey.

出版信息

Ulus Travma Acil Cerrahi Derg. 2003 Jul;9(3):163-8.

PMID:12923690

Abstract

BACKGROUND

Whether nitric oxide (NO) protects or impairs the liver function and structure during the early phase of sepsis is still controversial. The aim of this study is to evaluate the effect of NO on lipopolysaccharide (LPS) or lipoteichoic acid (LTA) induced liver injury in rats.

METHODS

One hundred twenty-six Wistar rats were assigned randomly and equally to LTA and LPS groups. Each group was divided into three subgroups which received saline, L-Arginine (L-Arg) or NG-nitro-L-arginine methylester (L-NAME) consecutively, one hour prior to either 5 mg/kg LPS or 3 mg/kg LTA injection. Plasma nitrite plus nitrate [NOx] were measured. Liver injury was assessed by measuring the rises in circulating liver enzymes and by scoring the extent of liver necrosis.

RESULTS

Administration of L-NAME+LPS not only reduced [NOx] production but also enhanced liver damage. LNAME+ LTA caused an increase in the plasma levels of [NOx] (p=0.0006), and produced sinusoidal enlargement in liver. L-Arg protected the hepatocytes against LPS injury, whereas, enhanced the liver damage in the LTA group.

CONCLUSION

Our data indicated that overproduction of NO exerts detrimental effect on LTA-treated rats while providing a protective function in LPS group.

摘要

背景

在脓毒症早期，一氧化氮（NO）对肝功能和结构是起保护作用还是造成损害仍存在争议。本研究旨在评估NO对脂多糖（LPS）或脂磷壁酸（LTA）诱导的大鼠肝损伤的影响。

方法

126只Wistar大鼠被随机且平均分为LTA组和LPS组。每组再分为三个亚组，在注射5mg/kg LPS或3mg/kg LTA前1小时，分别连续给予生理盐水、L-精氨酸（L-Arg）或NG-硝基-L-精氨酸甲酯（L-NAME）。检测血浆亚硝酸盐加硝酸盐[NOx]水平。通过测量循环肝酶升高情况及对肝坏死程度进行评分来评估肝损伤。