辐射与依托泊苷在体外对两种放射敏感性不同的细胞系的相互作用。
Interaction of radiation and etoposide on two cell lines with different radiosensitivities in vitro.
作者信息
Takahashi Takeo, Mitsuhashi Norio, Akimoto Tetsuo, Matsumoto Hiroko, Ishikawa Hitoshi, Saito Yoshihiro, Niibe Hideo
机构信息
Department of Radiology, Saitama Medical Center, Saitama Medical School 1981, Kamodatsujido-cho, Kawagoe, Saitama 350-8550, Japan.
出版信息
Anticancer Res. 2003 Jul-Aug;23(4):3459-64.
PURPOSE
To investigate the differences between two rat yolk sac tumor cell lines with different radiosensitivities in etoposide sensitivity and in the sensitizing effect of etoposide in combination with irradiation.
MATERIALS AND METHODS
NMT-1 is a parent radiosensitive cell line and NMT-1R is a variant radioresistant cell line. The effects were assessed by clonogenic assay. Apoptosis was evaluated by DNA fragmentation to investigate the mechanism of cell death.
RESULTS
Etoposide potentiation of radiation sensitivity was manifested by elimination of the initial shoulder of the cell survival curve in radioresistant NMT-1R cells. However, there was no enhancement effect in radiosensitive NMT-1 cells. The incidence of apoptosis was elevated in NMT-1R cells treated with etoposide and radiation.
CONCLUSION
Etoposide had a supra-additive effect in combination with irradiation for radioresistant NMT-1R cells due to the induction of apoptosis. However, a suppra-additive effect was not seen in radiosensitive NMT-1 cells.
目的
研究两种放射敏感性不同的大鼠卵黄囊瘤细胞系在依托泊苷敏感性以及依托泊苷与照射联合应用时的增敏作用方面的差异。
材料与方法
NMT-1是亲本放射敏感细胞系，NMT-1R是变异的放射抗性细胞系。通过克隆形成试验评估效果。通过DNA片段化评估凋亡以研究细胞死亡机制。
结果
在放射抗性的NMT-1R细胞中，依托泊苷对放射敏感性的增强表现为消除细胞存活曲线的初始肩部。然而，在放射敏感的NMT-1细胞中没有增强作用。用依托泊苷和照射处理的NMT-1R细胞中凋亡发生率升高。
结论
由于诱导凋亡，依托泊苷与照射联合应用对放射抗性的NMT-1R细胞具有超相加效应。然而，在放射敏感的NMT-1细胞中未观察到超相加效应。
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