Cabello P, Speleman F, Ferro M T, Resino M, García-Sagredo J M, San Román C, Burgaleta M C
Servicio de Genética Médica, Hospital Ramón y Cajal, Madrid.
Sangre (Barc). 1992 Dec;37(6):457-9.
The non-isotopic in situ hybridization makes it possible the analysis of, both, numeric and structural chromosome aberrations in interphase nuclei. Moreover, this technique is useful for identification of chromosome markers of unknown origin, frequently present in malignant diseases. In our case, the fluorescent in situ hybridization allowed us, in a CML patient in accelerated phase, to know the origin of a chromosome marker, and then, to state that the patient had a 21 trisomy added to the Philadelphia chromosome.
非同位素原位杂交使得分析间期核中的染色体数目和结构畸变成为可能。此外，该技术对于识别恶性疾病中常见的不明来源的染色体标记物很有用。在我们的案例中，荧光原位杂交使我们能够在一名加速期慢性粒细胞白血病患者中确定一个染色体标记物的来源，进而确定该患者除费城染色体外还存在21三体。
