异钩藤碱成分的薄层色谱-傅里叶变换表面增强拉曼光谱研究

[TLC-FT-SERS study on ingredients of Isrhynchophylline].

作者信息

Wang Yuan, Wang Song-ying, Zhao Yi-xue, Ren Gui-fen, Zi Feng-lan

机构信息

Chemistry Department, Capital Normol University, Beijing 100037, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2002 Feb;22(1):43-5.

PMID:12940023

Abstract

A new method for analysing the ingredients of Isrhynchophylline in Uncaria Rhynchophylla Jacks by thin layer chromatography (TLC) and the surface-enhanced Raman spectroscopy (SERS) is reported in this paper. The results show that the characteristic spectra bands of Isrhynchophylline situated at the thin layer with the amount of sample about 2.5 micrograms were obtained. The difference between SERS and solid spectra was found. Great enhancement of the 1,615 cm-1 spectral band was abstained. Molecule was absorbed in surface silver sol by pi electrons in phenyl and by pair of electrons in N together. An absorption model of Isrhynchophylline and silver sol was proposed. This method can be used to analyse the chemical ingredients with high sensitivity.

摘要

本文报道了一种采用薄层色谱（TLC）和表面增强拉曼光谱（SERS）分析钩藤中异钩藤碱成分的新方法。结果表明，当样品量约为2.5微克时，在薄层上获得了异钩藤碱的特征光谱带。发现了SERS光谱与固体光谱之间的差异。获得了1615 cm-1光谱带的显著增强。分子通过苯环中的π电子和氮原子中的孤对电子共同吸附在表面银溶胶上。提出了异钩藤碱与银溶胶的吸附模型。该方法可用于高灵敏度地分析化学成分。