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[透过电子显微镜看热带生物学]

[A view of tropical biology through the electron microscope].

作者信息

Hernández-Chavarría Francisco

机构信息

Facultad de Microbiología, Centro de Investigación en Estructuras Microscópicas, Universidad de Costa Rica, San José, Costa Rica.

出版信息

Rev Biol Trop. 2002 Sep-Dec;50(3-4):927-40.

Abstract

The first electron microscope in Costa Rica was a donation from the government of Japan through its International Cooperation Agency (JICA) in 1974. This donation made possible the consolidation of what was to become the University of Costa Rica's Electron Microscope Unit (UME). Within three years the first scientific papers were published, dealing with ultrastructural aspects of "Corn's rayado fino virus" and rotavirus, viral agent of human diarrhea. Subsequent papers out of the UME were published for the most part in the Journal of Tropical Biology, totaling at least 50 in that journal alone by the year 2000. With the recent acquisition of Energy Dispersive Spectrometer to coupled in transmission electron microscope and scanning electron microscope to X ray analysis, the data acquisition of the UME has been greatly enhanced, making possible to analyze both structure and elemental chemical composition in a specimen. Other applications of this new technology include studies of environmental pollution with heavy metals, such as comparative analysis of residues on leaves from urban areas and those on leaves from primary forest.

摘要

哥斯达黎加的第一台电子显微镜是1974年日本政府通过其国际合作机构(日本国际协力机构)捐赠的。这笔捐赠使后来成为哥斯达黎加大学电子显微镜室(UME)的机构得以巩固。三年内发表了首批科学论文,内容涉及“玉米细线条病毒”和轮状病毒(人类腹泻的病毒病原体)的超微结构方面。UME随后发表的论文大多发表在《热带生物学杂志》上,仅到2000年,该杂志上就至少发表了50篇。随着最近购置了用于透射电子显微镜和扫描电子显微镜与X射线分析联用的能量色散谱仪,UME的数据采集能力得到了极大提高,从而能够分析标本的结构和元素化学成分。这项新技术的其他应用包括对重金属环境污染的研究,例如对城市地区树叶和原始森林树叶上残留物的比较分析。

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