Lei Yixiong, Liu Yuqing
Department of Preventive Medicine, Guangzhou Medical College, Guangzhou 510182, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):260-1.
This study was aimed to explore the possible carcinogenic mechanisms of cadumium and its related compounds involved at the molecular level.
We investigated the alternations in expressions of the proto-oncogens c-fos and c-jun in BALB/c-3T3 cells induced by cadmium chloride (CdCl2), using differential RT-PCR protocol.
It was found that 2 hours after the cells were exposed to CdCl2, overexpression of an immediate early proto-oncogen c-fos was observed in BALB/c-3T3 cells treated with CdCl2 in concentrations ranging from 5 mumol/ml to 40 mumol/ml, and there was a significant dose-dependent relationship between the concentrations of CdCl2 and the levels of c-fos expression after normalization with beta-actin. In the same way, elevated expression of another immediate early proto-oncogen, c-jun, was induced with CdCl2 in concentrations from 20 to 40 mumol/ml.
The results seem to indicate that overexpressions of c-fos and c-jun are related to genotoxicity of CdCl2. These results might partly explain the cell transformation and carcinogenesis of cadmium.
本研究旨在从分子水平探讨镉及其相关化合物可能的致癌机制。
我们采用差异逆转录聚合酶链反应（RT-PCR）方法，研究了氯化镉（CdCl2）诱导的BALB/c-3T3细胞中原癌基因c-fos和c-jun表达的变化。
发现细胞暴露于CdCl2 2小时后，在浓度为5μmol/ml至40μmol/ml的CdCl2处理的BALB/c-3T3细胞中观察到即刻早期原癌基因c-fos的过表达，并且在用β-肌动蛋白标准化后，CdCl2浓度与c-fos表达水平之间存在显著的剂量依赖性关系。同样，浓度为20至40μmol/ml的CdCl2诱导了另一个即刻早期原癌基因c-jun的表达升高。
结果似乎表明c-fos和c-jun的过表达与CdCl2的遗传毒性有关。这些结果可能部分解释了镉的细胞转化和致癌作用。
