[氯化镉诱导BALB/c-3T3细胞中原癌基因c-fos和c-jun的表达]

[Induction of proto-oncogens c-fos and c-jun expression in BALB/c-3T3 cells by cadmium chloride].

作者信息

Lei Yixiong, Liu Yuqing

机构信息

Department of Preventive Medicine, Guangzhou Medical College, Guangzhou 510182, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):260-1.

PMID:12947706

Abstract

OBJECTIVE

This study was aimed to explore the possible carcinogenic mechanisms of cadumium and its related compounds involved at the molecular level.

METHODS

We investigated the alternations in expressions of the proto-oncogens c-fos and c-jun in BALB/c-3T3 cells induced by cadmium chloride (CdCl2), using differential RT-PCR protocol.

RESULTS

It was found that 2 hours after the cells were exposed to CdCl2, overexpression of an immediate early proto-oncogen c-fos was observed in BALB/c-3T3 cells treated with CdCl2 in concentrations ranging from 5 mumol/ml to 40 mumol/ml, and there was a significant dose-dependent relationship between the concentrations of CdCl2 and the levels of c-fos expression after normalization with beta-actin. In the same way, elevated expression of another immediate early proto-oncogen, c-jun, was induced with CdCl2 in concentrations from 20 to 40 mumol/ml.

CONCLUSION

The results seem to indicate that overexpressions of c-fos and c-jun are related to genotoxicity of CdCl2. These results might partly explain the cell transformation and carcinogenesis of cadmium.

摘要

目的

本研究旨在从分子水平探讨镉及其相关化合物可能的致癌机制。

方法

我们采用差异逆转录聚合酶链反应（RT-PCR）方法，研究了氯化镉（CdCl2）诱导的BALB/c-3T3细胞中原癌基因c-fos和c-jun表达的变化。

结果

发现细胞暴露于CdCl2 2小时后，在浓度为5μmol/ml至40μmol/ml的CdCl2处理的BALB/c-3T3细胞中观察到即刻早期原癌基因c-fos的过表达，并且在用β-肌动蛋白标准化后，CdCl2浓度与c-fos表达水平之间存在显著的剂量依赖性关系。同样，浓度为20至40μmol/ml的CdCl2诱导了另一个即刻早期原癌基因c-jun的表达升高。