Labudović Danica D, Toseska Katerina N, Alabakovska Sonja B, Dimitrovski Cedo, Jurhar Maja, Isjanovska Rozalinda, Spiroski Mirko, Todorova Bojana B
Department of Medical and Experimental Biochemistry, Medical Faculty, 50 Divizija Str. No. 6, 1000 Skopje, Republic of Macedonia.
Croat Med J. 2003 Aug;44(4):435-40.
To determine the frequency distribution of apoprotein(a) isoforms in patients with insulin-dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus and healthy subjects.
We separated and visualized 5 apo(a) isoforms in 40 patients with IDDM (12 men aged 48.00-/+4.59 and 28 women aged 52.37-/+8.21), 65 patients with NIDDM (26 men aged 61.88-/+9.25 and 39 women aged 60.15-/+7.98), and 182 healthy subjects, using 3-15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, followed by immunoblotting.
The frequency distribution of apo(a) isoforms was very similar in patients with diabetes mellitus and the control group. Atherogenic low molecular weight (LMW) S1 apo(a) isoform was more frequent in patients with IDDM (7.5%) and NIDDM (6.15%) than in the control group (0.78%). LMW S1 apo(a) isoform in patients with IDDM (relative risk [RR], 6.86; 95% confidence interval [CI], 1.19-25.21; p<0.001) and patients with NIDDM (RR, 7.04; 95% CI, 1.40-35.40; p=0.0057) as well as high molecular weight >S4 apo(a) isoform in patients with NIDDM (RR, 2.39; 95% CI, 1.28-5.21; p=0.0067) significantly increased the risk for the development of atherosclerosis. Mean molecular weight of S3, S1, and B apo(a) isoforms was higher in patients with IDDM and NIDDM than in the healthy subjects carriers of the same isoforms, but this difference was not statistically significant. We estimated high inverse statistical correlation between apo(a) size (kDa) and plasma lipoprotein(a) concentration in all study groups, patients with IDDM (p<0.001), patients with NIDDM (p<0.001), and healthy subjects (p<0.01).
Not only the increased plasma Lp(a) levels, but also apoprotein(a) isoforms may play an important role as a risk factor for the development of atherosclerosis in patients with diabetes mellitus.
确定胰岛素依赖型（IDDM）和非胰岛素依赖型（NIDDM）糖尿病患者及健康受试者中载脂蛋白（a）异构体的频率分布。
我们采用3 - 15%梯度十二烷基硫酸钠聚丙烯酰胺凝胶电泳，随后进行免疫印迹，对40例IDDM患者（12名男性，年龄48.00±4.59岁；28名女性，年龄52.37±8.21岁）、65例NIDDM患者（26名男性，年龄61.88±9.25岁；39名女性，年龄60.15±7.98岁）和182名健康受试者的5种载脂蛋白（a）异构体进行分离和可视化分析。
糖尿病患者和对照组中载脂蛋白（a）异构体的频率分布非常相似。致动脉粥样硬化的低分子量（LMW）S1载脂蛋白（a）异构体在IDDM患者（7.5%）和NIDDM患者（6.15%）中比对照组（0.78%）更常见。IDDM患者（相对危险度[RR]，6.86；95%可信区间[CI]，1.19 - 25.21；p<0.001）和NIDDM患者（RR，7.04；95%CI，1.40 - 35.40；p = 0.0057）中的LMW S1载脂蛋白（a）异构体以及NIDDM患者中的高分子量>S4载脂蛋白（a）异构体（RR，2.39；95%CI，1.28 - 5.21；p = 0.0067）显著增加了动脉粥样硬化发生的风险。IDDM和NIDDM患者中S3、S1和B载脂蛋白（a）异构体的平均分子量高于相同异构体的健康受试者携带者，但这种差异无统计学意义。我们估计在所有研究组中，包括IDDM患者（p<0.001）、NIDDM患者（p<0.001）和健康受试者（p<0.01），载脂蛋白（a）大小（kDa）与血浆脂蛋白（a）浓度之间存在高度负相关。
不仅血浆Lp（a）水平升高，载脂蛋白（a）异构体也可能作为糖尿病患者动脉粥样硬化发生的危险因素发挥重要作用。
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