Tan Qinggang, Ji Jian, Barbosa M A, Fonseca Carlos, Shen Jiacong
Department of Polymer Science, Zhejiang University, Hangzhou 310027, China.
Biomaterials. 2003 Nov;24(25):4699-705. doi: 10.1016/s0142-9612(03)00363-6.
Multilayer films consisting of polyethylenimine (PEI) and heparin were successfully prepared on biomedical 316L stainless steel surface via electrostatic self-assembly (ESA) of the PEI and heparin. The process of ESA of PEI/heparin was monitored by static contact angle, electrochemical impedance spectroscopy (EIS), reflection adsorption spectroscopy and X-ray photoelectron spectroscopy data. The contact angle and EIS data revealed that the multilayer coating was stable in Tris-HCl (pH 7.35) buffer solution for 21 days. The static platelet adhesion and static clotting time experiments indicated that the PEI/heparin-deposited stainless steel could resist the platelet adhesion and prolong the static clotting time effectively. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices.
通过聚乙烯亚胺（PEI）和肝素的静电自组装（ESA），在生物医学316L不锈钢表面成功制备了由PEI和肝素组成的多层膜。通过静态接触角、电化学阻抗谱（EIS）、反射吸附光谱和X射线光电子能谱数据监测PEI/肝素的ESA过程。接触角和EIS数据表明，多层涂层在Tris-HCl（pH 7.35）缓冲溶液中稳定21天。静态血小板粘附和静态凝血时间实验表明，PEI/肝素沉积的不锈钢可以抵抗血小板粘附并有效延长静态凝血时间。这种易于加工且与形状无关的方法可能在心血管装置的表面改性方面具有良好的潜力。
