Stich Oliver, Graus Francesc, Rasiah Christiane, Rauer Sebastian
Neurologische Klinik und Poliklinik der Albert-Ludwigs-Universitaet Freiburg, D-79106 Freiburg, Germany.
J Neuroimmunol. 2003 Aug;141(1-2):165-9. doi: 10.1016/s0165-5728(03)00257-1.
We investigated the presence of anti-Yo-specific oligoclonal antibody bands in cerebrospinal fluid (CSF) and serum samples of 9 patients with anti-Yo syndrome and 11 controls. Isoelectric focusing combined with affinity blotting, revealed anti-Yo-specific intrathecal antibody synthesis in all patients with anti-Yo syndrome: Four patients had positive anti-Yo-specific oligoclonal IgG bands in CSF which were not demonstrable in their sera; five CSF/serum pairs showed additional, more intensive, oligoclonal bands in CSF compared to the corresponding serum. Interestingly, four patients with absence of oligoclonal bands of total IgG in CSF revealed positive anti-Yo-specific oligoclonal bands in the same sample. This speaks for a higher sensitivity of detection of oligoclonal bands using an affinity blot loaded with Yo-specific antigen compared to an affinity blot coated with anti-human IgG used for the detection of oligoclonal bands of total IgG. In conclusion, the presence of anti-Yo-specific oligoclonal IgG bands in CSF which were absent, or less strong, in patients sera provides qualitative evidence of anti-Yo-specific IgG synthesis by intrathecal B-cell clones. These results could be of interest in detection of intrathecal-specific IgG synthesis in nervous system infectious diseases provided that the target antigen is known.
我们研究了9例抗Yo综合征患者及11名对照者的脑脊液（CSF）和血清样本中抗Yo特异性寡克隆抗体条带的存在情况。等电聚焦结合亲和印迹法显示，所有抗Yo综合征患者均存在抗Yo特异性鞘内抗体合成：4例患者脑脊液中抗Yo特异性寡克隆IgG条带呈阳性，而血清中未检测到；5对脑脊液/血清样本显示，与相应血清相比，脑脊液中额外出现了更强的寡克隆条带。有趣的是，4例脑脊液中总IgG寡克隆条带缺失的患者，在同一样本中抗Yo特异性寡克隆条带呈阳性。这表明，与用于检测总IgG寡克隆条带的抗人IgG包被的亲和印迹法相比，使用负载Yo特异性抗原的亲和印迹法检测寡克隆条带具有更高的灵敏度。总之，脑脊液中存在而患者血清中不存在或强度较弱的抗Yo特异性寡克隆IgG条带，为鞘内B细胞克隆合成抗Yo特异性IgG提供了定性证据。如果已知靶抗原，这些结果可能对检测神经系统感染性疾病中的鞘内特异性IgG合成具有重要意义。
