Chatel Guillaume, Montiel Grégory, Pré Martial, Memelink Johan, Thiersault Martine, Saint-Pierre Benoit, Doireau Pierre, Gantet Pascal
EA 2106, Biomolécules et Biotechologies Végétale, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, France.
J Exp Bot. 2003 Nov;54(392):2587-8. doi: 10.1093/jxb/erg275. Epub 2003 Sep 9.
A cDNA encoding a bHLH transcription factor was isolated by the yeast one-hybrid system from a Catharanthus roseus cDNA library using the G-box element of the Strictosidine synthase gene promoter as bait. The corresponding protein (named CrMYC1) was shown to bind specifically to the G-box in yeast. In C. roseus suspension cells CrMYC1 mRNA levels are induced by fungal elicitor and jasmonate suggesting that CrMYC1 may be involved in the regulation of gene expression in response to these signals.
利用酵母单杂交系统,以长春花碱合成酶基因启动子的G-盒元件为诱饵,从长春花cDNA文库中分离出一个编码bHLH转录因子的cDNA。相应的蛋白质(命名为CrMYC1)在酵母中被证明能特异性结合G-盒。在长春花悬浮细胞中,真菌激发子和茉莉酸可诱导CrMYC1 mRNA水平升高,这表明CrMYC1可能参与响应这些信号的基因表达调控。