Kuwahara Hiroshi, Kamei Jun-ichi, Nakamura Norihiro, Matsumoto Miho, Inoue Hiroki, Kanazawa Hiroshi
Department of Biological Science, Graduate School of Science, Osaka University, Machikaneyama, Toyonaka, Osaka 560-0043.
J Biochem. 2003 Aug;134(2):245-50. doi: 10.1093/jb/mvg137.
Calcineurin homologous protein (CHP) is an EF-hand Ca(2+)-binding protein capable of interacting with various cellular proteins including Na(+)/H(+) exchangers, kinesin-related proteins, and apoptosis-inducing protein kinase DRAK2. We investigated the role of CHP on the DRAK2 protein kinase in vitro. CHP significantly reduced (approximately 85% inhibition) the kinase activity of DRAK2 for both autophosphorylation and phosphorylation of exogenous substrate (myosin light chain). The inhibitory effect of CHP was dependent on the presence of Ca(2+), whereas the interaction between CHP and DRAK2 was not Ca(2+)-dependent. These observations suggest that CHP negatively regulates the apoptosis-inducing protein kinase DRAK2 in a manner that depends on intracellular Ca(2+)-concentration.
钙调神经磷酸酶同源蛋白（CHP）是一种EF手型Ca(2+)结合蛋白，能够与多种细胞蛋白相互作用，包括Na(+)/H(+)交换体、驱动蛋白相关蛋白和凋亡诱导蛋白激酶DRAK2。我们在体外研究了CHP对DRAK2蛋白激酶的作用。CHP显著降低了（约85%抑制率）DRAK2的自身磷酸化及外源底物（肌球蛋白轻链）磷酸化的激酶活性。CHP的抑制作用依赖于Ca(2+)的存在，而CHP与DRAK2之间的相互作用不依赖于Ca(2+)。这些观察结果表明，CHP以依赖于细胞内Ca(2+)浓度的方式对凋亡诱导蛋白激酶DRAK2进行负调控。
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