Wu Yuntao, Marsh Jon W
Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, Maryland 20892-4034, USA.
J Virol. 2003 Oct;77(19):10376-82. doi: 10.1128/jvi.77.19.10376-10382.2003.
Replication of human immunodeficiency virus (HIV) involves obligatory sequential processes. Following viral entry and reverse transcription, the newly synthesized viral DNA integrates into the host chromatin. Integration is mandatory for viral production, yet HIV infection of CD4 T cells in vivo results in high levels of nonintegrated DNA. The biological potential of nonintegrated HIV DNA is unclear; however, prior work has demonstrated a limited transcription of the nef gene by nonintegrated HIV in infected quiescent T-cell populations. In a kinetic analysis of HIV infection of metabolically active transformed and primary CD4 T cells, we find an unexpected transient expression of both early and late message by nonintegrated HIV DNA. However, only the early multiply spliced transcript was measurably translated. This restriction of protein expression was due in part to inadequate Rev function, since expression of Rev in trans resulted in the expression of the late structural gene gag by nonintegrated HIV DNA.
人类免疫缺陷病毒（HIV）的复制涉及一系列必然的连续过程。病毒进入细胞并完成逆转录后，新合成的病毒DNA会整合到宿主染色质中。整合对于病毒产生至关重要，但HIV在体内对CD4 T细胞的感染会导致大量未整合的DNA。未整合的HIV DNA的生物学潜能尚不清楚；然而，先前的研究表明，在受感染的静止T细胞群体中，未整合的HIV对nef基因有有限的转录。在对代谢活跃的转化型和原代CD4 T细胞进行HIV感染的动力学分析中，我们发现未整合的HIV DNA意外地短暂表达了早期和晚期信息。然而，只有早期多次剪接的转录本能够检测到翻译产物。这种蛋白质表达的限制部分是由于Rev功能不足，因为反式表达Rev会导致未整合的HIV DNA表达晚期结构基因gag。
