聚合酶链反应在结核性脑膜炎诊断中的效率

Efficiency of polymerase chain reaction for the diagnosis of tuberculous meningitis.

作者信息

Rafi Abdolnasser, Naghily Behroor

机构信息

Research Center for TB, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Southeast Asian J Trop Med Public Health. 2003 Jun;34(2):357-60.

PMID:12971562

Abstract

The early diagnosis of tuberculous meningitis (TBM) is very important. In this study, the efficiency of the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was compared with conventional methods (acid-fast microscopy and culture) for the detection of M. tuberculosis in cerebrospinal fluid(CSF) specimens from patients suspected of TBM. Of the 29 CSF specimens from highly-probable TBM patients (based on clinical features), 25 were positive by PCR (86.2%), whereas only one of 29 was acid-fast microscopy (AFM) positive (3.4%), and 5 out of 29 were culture-positive (17.2%). No positive results were found by AFM, culture or PCR in the non-tuberculous control group. The results of this study indicate that the application of PCR should be extremely useful in the diagnosis of TBM.

摘要

结核性脑膜炎（TBM）的早期诊断非常重要。在本研究中，将聚合酶链反应（PCR）（最可靠且灵敏的基于DNA的检测方法之一）的检测效率与传统方法（抗酸染色显微镜检查和培养）进行比较，以检测疑似TBM患者脑脊液（CSF）标本中的结核分枝杆菌。在29份来自高度疑似TBM患者（基于临床特征）的CSF标本中，25份PCR检测呈阳性（86.2%），而29份中只有1份抗酸染色显微镜检查（AFM）呈阳性（3.4%），29份中有5份培养阳性（17.2%）。在非结核对照组中，AFM、培养或PCR均未发现阳性结果。本研究结果表明，PCR的应用在TBM诊断中应极为有用。