Zimmer Manuel, Palmer Amparo, Köhler Jenny, Klein Rüdiger
Max-Planck Institute of Neurobiology, Am Klopferspitz 18A, D-82152 Martinsried, Germany.
Nat Cell Biol. 2003 Oct;5(10):869-78. doi: 10.1038/ncb1045. Epub 2003 Sep 14.
Eph receptors and their membrane-associated ephrin ligands mediate cell-cell repulsion to guide migrating cells and axons. Repulsion requires that the ligand-receptor complex be removed from the cell surface, for example by proteolytic processing of the ephrin ectodomain. Here we show that cell contact-induced EphB-ephrinB complexes are rapidly endocytosed during the retraction of cells and neuronal growth cones. Endocytosis occurs in a bi-directional manner that comprises of full-length receptor and ligand complexes. Endocytosis is sufficient to promote cell detachment and seems necessary for axon withdrawal during growth cone collapse. Here, we show a mechanism for the termination of adhesion and the promotion of cell repulsion after intercellular (trans) interaction between two transmembrane proteins.
Eph受体及其膜相关的ephrin配体介导细胞间排斥，以引导迁移细胞和轴突。排斥作用要求配体-受体复合物从细胞表面移除，例如通过对ephrin胞外域进行蛋白水解处理。我们在此表明，细胞接触诱导的EphB-ephrinB复合物在细胞和神经元生长锥回缩过程中迅速被内吞。内吞以双向方式发生，包括全长受体和配体复合物。内吞足以促进细胞脱离，并且似乎是生长锥塌陷期间轴突回缩所必需的。在此，我们展示了一种在两个跨膜蛋白之间进行细胞间（反式）相互作用后终止黏附并促进细胞排斥的机制。
