Ingwall J S, Morales M F, Stockdale F E, Wildenthal K
Recent Adv Stud Cardiac Struct Metab. 1975;8:467-81.
These experiments test whether creatine, a product of muscular contraction, stimulates myofibrillar protein synthesis. It was found that skeletal muscle cells formed both in vitro and in vivo and cardiac muscle cells formed in vivo synthesize myofibrillar proteins faster when supplied creatine in vitro. The rates of synthesis and/or accumulation of three myofibrillar proteins-myosin heavy chain actin, and creatine kinase-were stimulated by creatine. In contrast, the rates of synthesis of total protein and of deoxyribonucleic acid (DNA) and the activities of several nonmyofibrillar enzymes were not altered by creatine. These include lactic dehydrogenase, cathepsin D, acid phosphatase, and beta-acetylglucosaminidase. It is concluded that creatine selectively stimulated the rate of synthesis of contractile proteins in skeletal and cardiac muscle in vitro and may play a role in muscle hypertrophy.
这些实验旨在测试肌肉收缩产物肌酸是否能刺激肌原纤维蛋白合成。研究发现，体外培养的骨骼肌细胞以及体内形成的骨骼肌细胞和体内形成的心肌细胞，在体外供应肌酸时合成肌原纤维蛋白的速度更快。肌酸刺激了三种肌原纤维蛋白——肌球蛋白重链肌动蛋白和肌酸激酶的合成和/或积累。相比之下，总蛋白、脱氧核糖核酸（DNA）的合成速率以及几种非肌原纤维酶的活性并未因肌酸而改变。这些酶包括乳酸脱氢酶、组织蛋白酶D、酸性磷酸酶和β-乙酰氨基葡萄糖苷酶。得出的结论是，肌酸在体外能选择性地刺激骨骼肌和心肌中收缩蛋白的合成速率，并且可能在肌肉肥大中发挥作用。
